Showing papers in "Plant Science in 1993"
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TL;DR: This paper reviews the literature with the aim of presenting an updated status on some aspects of research on the responses opposed by plants to anaerobic stress and suggesting ways to avoid anoxia-induced biochemical changes that may be harmful to their life.
337 citations
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TL;DR: Embryogenic cells of asparagus were successfully cryopreserved by vitrification and subsequently regenerated plants via embryogenesis and plant regeneration efficiency was nearly the same as that of the untreated, unfrozen control.
273 citations
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TL;DR: Analysis of the characteristics of chlorophyll fluorescence induction in sub- and super-saturating light revealed two targets of mild heat stress: an irreversible inhibition of electron donation to PSII and a reversible reduction of excitation energy trapping by the PSII reaction centers, with the former effect being identified as the major determinant of the loss of photosynthesis.
241 citations
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TL;DR: Bioassays to test disease resistance of R1 progeny indicate that, compared to transgenic control and SB-37 plants, Shiva-1 seedlings exhibited delayed wilt symptoms and reduced disease severity and mortality after infection with a highly virulent strain of Pseudomonas solanacearum.
204 citations
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TL;DR: Elevated expression attributable to the AMV leader sequence was independent of abundance of the corresponding AMV- gus transcript, suggesting a post-transcriptional mechanism of action in vivo.
164 citations
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TL;DR: The data indicate that the root ZR level of N-limited barley is largely non-responsive to nitrate dose in the long term, but transiently responding (positively) to increased nitrate supply.
129 citations
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123 citations
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TL;DR: It is concluded that oleosins, such as the 22-kDa polypeptide in olive, are present in the long-term storage oil bodies from the embryo and endosperm tissues of the seed and are absent from oil bodies of the mesocarp.
118 citations
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TL;DR: Assessment of the extent of genetic diversity detected by RAPD (random amplified polymorphic DNA) technique among 15 varieties of common bread wheat revealed a dendrogram suggesting genetic relationships among these genotypes, useful in the identification of suitable parents for the development of a mapping population for tagging agronomically important traits in wheat.
116 citations
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TL;DR: The presence and integration of foreign DNA in hygromycin-resistant callus lines and regenerated plants has been confirmed by polymerase chain reaction amplification of a defined portion of the chimeric gene and by Southern hybridization analysis.
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TL;DR: Fertile transgenic maize plants were regenerated after direct transfer of a chimeric gene into maize protoplasts and molecular evidences based on Southern data and PCR analysis have indicated that the introduced gene was transferred in the first sexual generation.
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TL;DR: Thionins, which were not particularly effective in liquid medium against Phytophthora infestans, were more effective than the fungicide Ridomil on a molar basis in a drop application assay on leaf discs from potato.
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TL;DR: The nuclear DNA content of cells from individual organs of seedlings and plants of Cucumis sativus L. Hokus was determined during development by flow cytometry and revealed polysomaty, both specific to the developmental stage of the plant and to the individual organ.
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TL;DR: A high level of adventitious shoot regeneration was obtained from proliferating shoots in vitro for a range of Prunus spp.
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TL;DR: A procedure for increased efficiency of production of transgenic flax plants was developed by increasing in the cellular transformation intensity on inoculated hypocotyls, judicious choice of selection agent and optimization of selection scheme.
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TL;DR: Transgenic sweet potato plants obtained after Agrobacterium rhizogenes -mediated transformation had wrinkled leaves, altered shape of flowers, reduced apical dominance, shortened internodes, small storage roots and abundant, frequently branching roots that showed reduced geotropism.
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TL;DR: Addition of 10–25 mM proline with 0.4 mM thioproline and 50 mM potassium enhanced the size of embryogenic callus and significantly enhanced subsequent embryo formation along with higher numbers of cotyledonary embryos.
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TL;DR: Southern blot analysis clearly showed multiple integration of T-DNA (transferred DNA) and the presence of neomycin phosphotransferase (NPTII) gene sequences in the transformed black locust genome.
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TL;DR: A RAPD analysis provided evidences for limited newly-induced genetic variation among independent protoplast-derived ryegrass plants, similar behavior and cultivar-dependence was apparent when comparing plant regeneration directly from embryogenic suspension cultures with regeneration from corresponding protoplasts.
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TL;DR: An in vitro regeneration protocol was developed in chilli from immature zygotic embryos via direct somatic embryogenesis without involving intermediate callus and showed cytological and morphological uniformity.
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TL;DR: A concentration of 10−4M DON appeared to be the optimum concentration to differentiate between haploid wheat calli for DON tolerance, however, growth analysis data of 40 callus clones did not show any correlation with the known FHB resistance levels of the original donor genotypes and populations.
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TL;DR: Six types of somatic embryos differentiated from immature cotyledonary explants in peanut exhibited very divergent morphologies, and lower conversion rates in some classes were associated with poorly developed shoot meristematic areas.
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TL;DR: Evaluated the effects of duration in induction medium on calli induction, plant regeneration, and green plant production from anther cultures in spring wheat to identify a combination of 2,4-dichlorophenoxyacetic acid and IAA concentrations which produce the maximum number of haploid plants.
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TL;DR: It is shown that among the four compounds studied, MDA appears as the most reliable indicator of cold sensitivity for the two coffee species studied and its level increased in microcuttings of C. canephora stored at 12°C.
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TL;DR: A procedure for plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.) via somatic embryogenesis is described, and induced calli grew into plantlets which showed sustained growth on the MS medium containing only 0.1 M sorbitol as the sole carbon source.
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TL;DR: The activity of the biosynthetic genes correlated closely with the flavonoid content of the petals, with the synthesis of flavonols being temporally separated from that of anthocyanins.
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TL;DR: It is suggested that Ca2+-, PS- and PA-dependent or cAMP-dependent protein kinase in the rice leaf might be involved in cell regulatory systems through phosphorylation.
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TL;DR: DNA transfer into microspores and pollen was significantly greater in electroporated microspore than in non-treatedmicrospores, the first step towards transgenic plants obtained by the haploid way.