S
Sandra L. Spurgeon
Researcher at Applied Biosystems
Publications - 11
Citations - 693
Sandra L. Spurgeon is an academic researcher from Applied Biosystems. The author has contributed to research in topics: Acceptor & Three prime untranslated region. The author has an hindex of 6, co-authored 11 publications receiving 692 citations.
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Patent
Energy transfer dyes with enhanced fluorescence
TL;DR: In this paper, a novel linker for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has been proposed, which has the general structure R21Z1C(O)R22R28 where R21 is a C 1-5 alkyl attached to the donor dye, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to a carbonyl carbon, and R28
Journal ArticleDOI
New energy transfer dyes for DNA sequencing
Linda G. Lee,Sandra L. Spurgeon,C. R. Heiner,Scott C. Benson,Barnett B. Rosenblum,Steven M. Menchen,Ronald J. Graham,A. Constantinescu,Krishna G. Upadhya,Jonathan M. Cassel +9 more
TL;DR: A set of four energy transfer dyes are synthesized and demonstrated their use in automated DNA sequencing and their values were reduced by 20-25% compared with the dichlororhodamine dyes alone.
Journal ArticleDOI
Evolutionary conservation of the AU-rich 3' untranslated region of messenger RNA.
TL;DR: The results provide support for the growing body of evidence that 3' AURE are both conserved and functional and indicate further that isolation and short-term in vitro culture of sea urchin coelomocytes is sufficient to induce the expression of transcripts containing 3'AURE.
Patent
Method for drying dye-terminator sequencing reagents
TL;DR: In this article, a method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided, and a method is provided for performing a reaction on a nucleic acid substrate.
Patent
Methods of analyzing polynucleotides employing energy transfer dyes
TL;DR: In this paper, a novel linker for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has been proposed, which has the general structure R21Z1C(O)R22R28 where R21 is a C 1-5 alkyl attached to the donor dye, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to a carbonyl carbon, and R28