Savannah J. West

TL;DR: Results demonstrate that spectral imaging approaches can be used to assess cAMP gradients—and in general gradients in fluorescence and FRET—within intact cells, and demonstrate that 2D imaging studies of localized fluorescence signals and, in particular, cAMP signals may lead to erroneous conclusions about the existence and/or magnitude ofgradients in either FRET or the underlying cAMP messages.

TL;DR: Acyl-RAC as discussed by the authors is a recently developed method based on selective capture of endogenously S-acylated proteins by thiol-reactive Sepharose beads, which requires fewer steps and can yield more reliable results when coupled with mass spectrometry for identification of novel Sacylation targets.

TL;DR: In this article, the authors show that Orai1 is rapidly S-acylated at cysteine 143 upon ER Ca2+ store depletion, leading to the formation of activated CRAC channels.

TL;DR: In this paper, the authors demonstrate that DHHC21, a member of the DHHC family of mammalian protein acyltransferases, mediates T cell receptor-induced S-acylation of proximal T cell signaling proteins.

TL;DR: In this paper , it was shown that S-acylation of Orai1 is dynamically regulated in a stimulus-dependent manner and essential for its function as a calcium channel.