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Sebastian van de Linde

Researcher at University of Strathclyde

Publications -  60
Citations -  7232

Sebastian van de Linde is an academic researcher from University of Strathclyde. The author has contributed to research in topics: Fluorescence-lifetime imaging microscopy & Microscopy. The author has an hindex of 31, co-authored 56 publications receiving 6465 citations. Previous affiliations of Sebastian van de Linde include University of Würzburg & Bielefeld University.

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Direct stochastic optical reconstruction microscopy with standard fluorescent probes

TL;DR: A step-by-step protocol for dSTORM imaging in fixed and living cells on a wide-field fluorescence microscope, with standard fluorescent probes focusing especially on the photoinduced fine adjustment of the ratio of fluorophores residing in the ON and OFF states is presented.
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Light-induced cell damage in live-cell super-resolution microscopy.

TL;DR: This work applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode, wavelength, light-dose, temperature and fluorescence labeling on the survival probability of different cell lines 20–24 hours after irradiation.
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rapidSTORM: accurate, fast open-source software for localization microscopy.

TL;DR: Besides being versatile and fast, rapidSTORM is easy to use, deploy, inspect and extend, and based on widespread, mature, portable and open technologies such as C++, the GNU tool chain and wxWidgets.