S
Sebastian van de Linde
Researcher at University of Strathclyde
Publications - 60
Citations - 7232
Sebastian van de Linde is an academic researcher from University of Strathclyde. The author has contributed to research in topics: Fluorescence-lifetime imaging microscopy & Microscopy. The author has an hindex of 31, co-authored 56 publications receiving 6465 citations. Previous affiliations of Sebastian van de Linde include University of Würzburg & Bielefeld University.
Papers
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Journal ArticleDOI
Correction to: Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging
Journal ArticleDOI
Benchmarking Thiolate-Driven Photoswitching of Cyanine Dyes
TL;DR: In this article , the role of thiols in cyanine photoswitching at varying intensities generated in a single acquisition by a microelectromechanical systems (MEMS) mirror placed in the excitation path was investigated.
Journal ArticleDOI
Single‐Molecule Photoswitching and Localization
TL;DR: In this paper, the principles and requirements of single-molecule based localization microscopy, and compare different super-resolution imaging concepts and highlight their strengths and limitations with respect to applications in fixed and living cells with high spatio-temporal resolution.
Journal ArticleDOI
dSTORM of Synaptic Proteins
Markus Sauer,Sebastian van de Linde,Thorge Holm,Sarah Aufmkolk,Nadine Ehmann,Robert Blum,Dana Bar-On,Amit Alon,Uri Ashery,Dimitrij Ljaschenko,Manfred Heckmann,Robert J. Kittel +11 more
TL;DR: How super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy (dSTORM) can be used advantageously to study the distribution of synaptic proteins with an optical resolution of 15-20 nm in the imaging plane using standard, commercially available fluorescent probes is demonstrated.
Proceedings ArticleDOI
Buffer controlled photoswitching microscopy using standard organic fluorophores
Volker Buschmann,Samantha Fore,Sebastian van de Linde,Markus Sauer,Steve Wolter,Mike Heilemann,Felix Koberling,Rainer Erdmann +7 more
TL;DR: In this article, the authors exploited this redox-level adjusted photoswitching behavior based on addition of millimolar amounts of reducing enzymes and reducing enzymes for high-resolution imaging on a setup based on an inverse microscope coupled with an ultrasensitive CCD camera.