Seiichi Yasuda

TL;DR: Two independent mutants of Escherichia coli lacking murein-lipoprotein have been found in this paper, one of which was named lpo and the other was known as lpo.

TL;DR: The replication origin of Escherichia coli has been cloned on a nonreplicating DNA fragment coding for ampicillin resistance and is concluded that the replication origin is located at a site between uncA and rbsK, at about 83 min on the genetic map of E. coli.

TL;DR: Since mutants defective in function for autonomous replication have been isolated in the corresponding positions of the common sequence at each binding site, dnaA protein-binding at these sites seems to be significant for replication initiation.

TL;DR: In this paper, a collection of 2000 Escherichia coli strains which harbor ColE1 plasmids carrying small random segments of the E. coli chromosome was screened for the correction of mutational defects in penicillin-binding proteins (PBPs): ponA (PBP-1a), ponB(PBP1b), dacB (pBP-4), and pfv (Pfv) were analyzed.

TL;DR: Results suggested that the β′ subunit of Escherichia coli K-12 had an essential role for promoter selection and at high temperature, the initiation of RNA synthesis was impaired and the degree of inhibition was different with templates used.