S
Shinji Kakimoto
Researcher at Osaka City University
Publications - 6
Citations - 132
Shinji Kakimoto is an academic researcher from Osaka City University. The author has contributed to research in topics: Gene delivery & Transfection. The author has an hindex of 4, co-authored 6 publications receiving 124 citations.
Papers
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Journal ArticleDOI
The conjugation of diphtheria toxin T domain to poly(ethylenimine) based vectors for enhanced endosomal escape during gene transfection.
Shinji Kakimoto,Tsutomu Hamada,Yuuki Komatsu,Masahiro Takagi,Toshizumi Tanabe,Hideki Azuma,Seiji Shinkai,Takeshi Nagasaki +7 more
TL;DR: The conjugation of diphtheria toxin T domain with PEI/pDNA polyplex leads to the significant enhancement of transfection efficiency when compared with plain PEI-conjugated poly(ethylenimine)s (PEI) polyplex.
Journal ArticleDOI
In vitro gene delivery to HepG2 cells using galactosylated 6-amino-6-deoxychitosan as a DNA carrier
TL;DR: The results suggest that the enhancement of transfection efficiency of Gal-6ACT was not due to the increase of receptor-mediated cellular uptake, and the enhanced gene transfer efficiency was not specific to the galactose modification.
Journal ArticleDOI
Dual-ligand effect of transferrin and transforming growth factor alpha on polyethyleneimine-mediated gene delivery.
TL;DR: The enhancement of internalization efficiency by dual ligands is an important factor for improving transfection efficiency and depends on cell species; receptor-mediated and efficient internalization may be related to this enhanced transfections efficiency.
Journal ArticleDOI
Enhanced internalization and endosomal escape of dual-functionalized poly(ethyleneimine)s polyplex with diphtheria toxin T and R domains.
TL;DR: It was demonstrated that DTRS-polyplex had high endosomal escape efficiency and internalization efficiency by several measurements, such as in vitro intracellular trafficking observation and the internalization inhibition with several inhibitors.
Patent
Nucleic acid construct
TL;DR: In this paper, a nucleic acid construct for nuclear import is presented, which comprises a ternary complex consisting of a nucleis acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for instance, polyethyleneimine) bound to both of the nucleisacid substance and the importin proteins.