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Shinobu Tsuchiaka
Researcher at Tokyo University of Agriculture and Technology
Publications - 30
Citations - 502
Shinobu Tsuchiaka is an academic researcher from Tokyo University of Agriculture and Technology. The author has contributed to research in topics: Virus & Population. The author has an hindex of 12, co-authored 30 publications receiving 394 citations. Previous affiliations of Shinobu Tsuchiaka include Gifu University.
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Journal ArticleDOI
Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
Mai Kishimoto,Shinobu Tsuchiaka,Shinobu Tsuchiaka,Sayed Samim Rahpaya,Sayed Samim Rahpaya,Ayako Hasebe,Keiko Otsu,Satoshi Sugimura,Suguru Kobayashi,Natsumi Komatsu,Makoto Nagai,Makoto Nagai,Tsutomu Omatsu,Tsutomu Omatsu,Yuki Naoi,Kaori Sano,Sachiko Okazaki-Terashima,Mami Oba,Yukie Katayama,Reiichiro Sato,Tetsuo Asai,Tetsuya Mizutani,Tetsuya Mizutani +22 more
TL;DR: A detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run to show high sensitivity and wide detection targets.
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Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus
Makoto Nagai,Tsutomu Omatsu,Hiroshi Aoki,Konosuke Otomaru,Takehiko Uto,Motoya Koizumi,Fujiko Minami-Fukuda,Hikaru Takai,Toshiaki Murakami,Tsuneyuki Masuda,Hiroshi Yamasato,Mai Shiokawa,Shinobu Tsuchiaka,Yuki Naoi,Kaori Sano,Sachiko Okazaki,Yukie Katayama,Mami Oba,Tetsuya Furuya,Junsuke Shirai,Tetsuya Mizutani +20 more
TL;DR: A viral metagenomics approach was used to investigate fecal samples of Japanese calves with and without diarrhea, and one of 15 strains exhibited low amino acid sequence similarity to other BoAstVs and was clustered separately with porcine astrovirus type 5 in all trees, and ovineAstrovirus in the ORF2 region, suggesting past interspecies transmission.
Journal ArticleDOI
Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination.
Mika Ito,Moegi Kuroda,Tsuneyuki Masuda,Masataka Akagami,Kei Haga,Shinobu Tsuchiaka,Mai Kishimoto,Yuki Naoi,Kaori Sano,Tsutomu Omatsu,Yukie Katayama,Mami Oba,Hiroshi Aoki,Toru Ichimaru,Itsuro Mukono,Yoshinao Ouchi,Hiroshi Yamasato,Junsuke Shirai,Kazuhiko Katayama,Tetsuya Mizutani,Makoto Nagai,Makoto Nagai +21 more
TL;DR: An analysis of nearly complete genomes of 36 PoAstVs detected by a metagenomics approach in the feces of Japanese pigs revealed evidence of multiple possible intra-genotype recombination events in PoAstV2 andPoAstV4, suggesting that recombination might have contributed to the genetic diversity and played an important role in the evolution of JapanesePoAstVs.
Journal ArticleDOI
Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.
Tsuneyuki Masuda,Makoto Nagai,Hiroshi Yamasato,Shinobu Tsuchiaka,Sachiko Okazaki,Yukie Katayama,Mami Oba,Naomi Nishiura,Yukiko Sassa,Tsutomu Omatsu,Tetsuya Furuya,Satoshi Koyama,Junsuke Shirai,Koki Taniguchi,Yoshiki Fujii,Reiko Todaka,Kazuhiko Katayama,Tetsuya Mizutani +17 more
TL;DR: The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that Tottori-SG had an unreported genotype combination G15P, suggesting that it might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle.
Journal Article
Development of one-step real-time reverse transcriptase-PCR-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea.
Tsuneyuki Masuda,Shinobu Tsuchiaka,Tomoko Ashiba,Hiroshi Yamasato,Kazuhiro Fukunari,Tsutomu Omatsu,Tetsuya Furuya,Junsuke Shirai,Tetsuya Mizutani,Makoto Nagai +9 more
TL;DR: qPCR can drastically reduce the diagnostic time to detect viruses compared to currently employed methods, and it is predicted that the qPCR assays will become a useful tool for detecting viral infections that cause diarrhea and other complications in pigs.