Showing papers by "Shiro Suzuki published in 2014"
TL;DR: The progress of microbial production of plant specialized metabolites, including phenylpropanoids, flavonoids, stilbenoids, diarylheptanoids, phenylbutanoid, terpenoids, and alkaloids, is described, and future challenges in this field are discussed.
Abstract: Plant specialized metabolites play important roles in human life. These metabolites, however, are often produced in small amounts in particular plant species. Moreover, some of these species are endangered in their natural habitats, thus further limiting the availability of some plant specialized metabolites. Microbial production of these compounds may circumvent this problem. Considerable progress has been made in the microbial production of various plant specialized metabolites over the past decade. Now, the microbial production of these compounds is becoming robust, fine-tuned, and commercially relevant systems using the methods of synthetic biology. This review describes the progress of microbial production of plant specialized metabolites, including phenylpropanoids, flavonoids, stilbenoids, diarylheptanoids, phenylbutanoids, terpenoids, and alkaloids, and discusses future challenges in this field.
17 citations
TL;DR: Analysis of the enantiomeric composition of products from racemic matairesinol showed all OMT reactions to be highly selective in terms of the substrate enantiomers and only use the (−)-enantiomer as the substrate.
Abstract: Previously we reported a cDNA encoding an O-methyltransferase (OMT) responsible for the O-methylation of matairesinol to afford arctigenin in Carthamus tinctorius. However, the regioselectivity and stereoselectivity of its reaction are not yet well understood. In this paper, we report the characterization and comparison of three matairesinol OMTs (MROMTs) encoded by cDNAs isolated from C. tinctorius (CtMROMT), Anthriscus sylvestris (AsMROMT), and Forsythia koreana (FkMROMT). Although they shared matairesinol as a substrate, each recombinant MROMT showed different catalytic behavior. AsMROMT and CtMROMT methylated matairesinol’s hydroxyl group at the C4′ position giving rise to arctigenin, while FkMROMT methylated the C4 position hydroxyl group giving rise to isoarctigenin. Analysis of the enantiomeric composition of products from racemic matairesinol showed all OMT reactions to be highly selective in terms of the substrate enantiomers and only use the (−)-enantiomer as the substrate.
16 citations
TL;DR: The detrimental effects on tree growth are often concomitant with the metabolic engineering of wood components, so fine-tuned regulation of transgene expression, and the production of value-added products may be targeted in future forest tree biotechnology.
Abstract: Forest trees produce an important feedstock, wood Forest tree breeding programs have been traditionally carried out by selecting elite trees to enhance productivity and processability Recently, however, a biotechnological approach has attracted much attention because it enables efficient and versatile improvement of forest trees In the last decade, forest tree biotechnology has considerably progressed: genomic sequences of several forest tree species have been decoded, efficient Agrobacterim-mediated genetic transformation and regeneration systems have been established in a number of forest tree species, and many reports have been published on the metabolic engineering of a major wood component, lignin, in forest trees However, in contrast to the metabolic engineering of lignin, the metabolic engineering of cellulose and hemicelluloses in forest trees awaits further development The detrimental effects on tree growth are often concomitant with the metabolic engineering of wood components To mitigate such effects, fine-tuned regulation of transgene expression, and the production of value-added products may be targeted in future forest tree biotechnology
8 citations
20 Oct 2014
TL;DR: Yamamura et al. as mentioned in this paper characterized cell wall phenylpropanoids of grass bioenergy plants and characterized ubiquitin ligase involved in secondary cell wall formation in Arabidopsis.
Abstract: Characterization of cell wall phenylpropanoids of grass bioenergy plants and characterization of ubiquitin ligase involved in secondary cell wall formation in Arabidopsis Author(s) Yamamura, Masaomi; Suzuki, Shiro; Umezawa, Toshiaki Citation Sustainable humanosphere : bulletin of Research Institute for Sustainable Humanosphere Kyoto University (2014), 10: 3-3 Issue Date 2014-10-20 URL http://hdl.handle.net/2433/196703 Right Type Departmental Bulletin Paper Textversion publisher
1 citations