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Shixin Liu
Researcher at Rockefeller University
Publications - 64
Citations - 1995
Shixin Liu is an academic researcher from Rockefeller University. The author has contributed to research in topics: Medicine & Chromatin. The author has an hindex of 20, co-authored 48 publications receiving 1373 citations. Previous affiliations of Shixin Liu include Harvard University & University of Science and Technology of China.
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Journal ArticleDOI
Optical tweezers in single-molecule biophysics.
TL;DR: The physical principles of optical tweezers and the characteristics that make them a powerful tool to investigate single molecules are reviewed, followed by a survey of applications of these methods to the studies of protein-nucleic acid interactions, protein/RNA folding and molecular motors.
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Slide into Action: Dynamic Shuttling of HIV Reverse Transcriptase on Nucleic Acid Substrates
Shixin Liu,Elio A. Abbondanzieri,Jason W. Rausch,Stuart F. J. Le Grice,Xiaowei Zhuang,Xiaowei Zhuang +5 more
TL;DR: Single-molecule fluorescence resonance energy transfer was used to probe the interactions between RT and nucleic acid substrates in real time and found that RT can spontaneously flip into a polymerization orientation, including normal DNA polymerization and strand displacement synthesis.
Journal ArticleDOI
A Viral Packaging Motor Varies Its DNA Rotation and Step Size to Preserve Subunit Coordination as the Capsid Fills
Shixin Liu,Shixin Liu,Gheorghe Chistol,Craig L. Hetherington,Craig L. Hetherington,Sara Tafoya,K. Aathavan,Joerg Schnitzbauer,Joerg Schnitzbauer,Shelley Grimes,Paul J. Jardine,Carlos Bustamante +11 more
TL;DR: This study investigates a viral packaging machine as it fills the capsid with DNA and encounters increasing internal pressure and finds that the motor rotates the DNA during packaging and that the rotation per base pair increases with filling.
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Complete dissection of transcription elongation reveals slow translocation of RNA polymerase II in a linear ratchet mechanism
Manchuta Dangkulwanich,Toyotaka Ishibashi,Toyotaka Ishibashi,Shixin Liu,Maria L. Kireeva,Lucyna Lubkowska,Mikhail Kashlev,Carlos Bustamante +7 more
TL;DR: Surprisingly, it is found that the forward translocation rate is comparable to the catalysis rate, revealing a linear, non-branched ratchet mechanism for the nucleotide addition cycle in which translocation is one of the rate-limiting steps.
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High Degree of Coordination and Division of Labor among Subunits in a Homomeric Ring ATPase
Gheorghe Chistol,Shixin Liu,Craig L. Hetherington,Jeffrey R. Moffitt,Shelley Grimes,Paul J. Jardine,Carlos Bustamante,Carlos Bustamante +7 more
TL;DR: A comprehensive mechanochemical characterization of a homomeric ring ATPase-the bacteriophage φ29 packaging motor-a homopentamer that translocates double-stranded DNA in cycles composed of alternating dwells and bursts and shows that the motor displays an unexpected division of labor.