M
Michelle D. Wang
Researcher at Howard Hughes Medical Institute
Publications - 115
Citations - 10368
Michelle D. Wang is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: RNA polymerase & DNA. The author has an hindex of 41, co-authored 109 publications receiving 9300 citations. Previous affiliations of Michelle D. Wang include Princeton University & Cornell University.
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Stretching DNA with optical tweezers
TL;DR: Force-extension (F-x) relationships were measured for single molecules of DNA under a variety of buffer conditions, using an optical trapping interferometer modified to incorporate feedback control, suggesting that the intrinsic persistence length remains close to 40 nm.
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Force and Velocity Measured for Single Molecules of RNA Polymerase
TL;DR: Modeling the data suggests that high loads may halt RNAP by promoting a structural change which moves all or part of the enzyme backwards through a comparatively large distance, corresponding to 5 to 10 base pairs, contrasts with previous models that assumed force acts directly upon a single-base translocation step.
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Estimating the persistence length of a worm-like chain molecule from force-extension measurements.
C. Bouchiat,Michelle D. Wang,Jean-François Allemand,Terence R. Strick,Steven M. Block,Vincent Croquette +5 more
TL;DR: An improvement to the Marko and Siggia interpolation formula of Bustamante et al (Science 1994, 265:1599-1600) that is useful for fitting experimental data is applied to the experimental elasticity curve of single DNA molecules.
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Transcription against an applied force
TL;DR: The force produced by a single molecule of Escherichia coli RNA polymerase during transcription was measured optically and is substantially larger than those measured for the cytoskeletal motors kinesin and myosin and exceeds mechanical loads that are estimated to oppose transcriptional elongation in vivo.
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Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
Brent D. Brower-Toland,Carolyn L. Smith,Richard C. Yeh,John T. Lis,Craig L. Peterson,Michelle D. Wang +5 more
TL;DR: The dynamic structure of individual nucleosomes was examined by stretching nucleosomal arrays with a feedback-enhanced optical trap and when arrays were relaxed before the dissociated state was reached, nucleosome were able to reassemble and to repeat the disassembly process.