Showing papers by "Simone Simionatto published in 2012"
TL;DR: Both cellular and humoral immune responses were induced and P42 and P95 are promising candidates for a recombinant subunit vaccine and P46 is a promising candidate for a DNA vaccine against PEP.
29 citations
TL;DR: Several recombinant proteins were specifically recognized by convalescent pig sera, indicating they are expressed during infection, and may help to develop more efficacious vaccines against M. hyopneumoniae.
Abstract: Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, is highly prevalent worldwide and causes major economic losses to the pig industry. Commercial vaccines are widely used in the control of this disease, however, they provide only partial protection. The aim of this study was to evaluate 34 recombinant proteins of M. hyopneumoniae expressed in Escherichia coli. Antigenic and immunogenic properties of these proteins were analyzed. For this, the proteins were tested against hyperimmune and convalescent pig sera through ELISA and Western blot. Immunogenicity of the recombinant proteins was evaluated in BALB/c mice following intramuscular inoculation. Most antigens were able to induce a strong immune response and sera from inoculated mice were able to recognize native proteins by cell ELISA and Western blot. Several recombinant proteins were specifically recognized by convalescent pig sera, indicating they are expressed during infection. These data may help to develop more efficacious vaccines against M. hyopneumoniae.
26 citations
TL;DR: Nine genes coding for transmembrane proteins from M. hyopneumoniae were cloned and six were successfully expressed in Escherichia coli and had their immunogenic and antigenic properties evaluated.
17 citations