Stefano Menzo

TL;DR: 5-year follow-up surveillance shows that CRF60_BC gained epidemic importance, spreading among young MSM in multiple Italian regions and increasing its population size in few years, as the number of sequences identified so far has triplicated since the authors' first report.

TL;DR: An easy and reliable method for the construction and quantification of competitive templates obtained as recombinant PCR products was developed and the technique was used for the absolute quantifying of human genomic DNA with primers from a single copy, subtelomeric region of chromosome 19.

TL;DR: A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized and proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA.

TL;DR: Genomic sequencing of the viral isolate showed that the virus belonged to WNV lineage 2, and detected WNV-specific IgM and IgG antibodies in the patient's serum.

TL;DR: Interestingly, despite the increasing viremia level associated with infection progression, the mean transcriptional activity of individual infected cells was found to be only moderately greater in AIDS patients than in asymptomatic infected subjects, and it was noted that quantitation of HIV-1 genomic RNA in plasma samples and quantitation in peripheral blood mononuclear cells appear to be more reliable and sensitive markers of viral activity than quantitative analysis of proviral HIV- 1 sequences in peripheral lymphocytes.