Stephen C.Y. Ip

TL;DR: Chemical crosslinking experiments reveal that these lymphocytes express novel T3-associated polypeptides, one of which appears to be the product of the Tγ gene.

TL;DR: The human Holliday junction resolvase, GEN1, and its yeast orthologue, Yen1, were independently identified using two distinct experimental approaches: GEN1 was identified by mass spectrometry following extensive fractionation of HeLa cell-free extracts, whereas Yen1 was detected by screening a yeast gene fusion library for nucleases capable of Holliday junctions resolution.

TL;DR: This work provides the first biochemical/structural characterization of GEN1, showing that, like the Escherichia coli HJ resolvase RuvC, it binds specifically to HJs and resolves them by a dual incision mechanism in which nicks are introduced in the pair of continuous strands within the lifetime of the GEN1-HJ complex.

TL;DR: It is proposed that TRF2 contributes to t‐loop stabilisation by stimulating HJ formation and by preventing resolvase cleavage, and this findings provide novel insights into the interplay between telomere protection and homologous recombination.

TL;DR: It is reported that chromosome unlinking can instead be accomplished by multiple rounds of site‐specific recombination, and that FtsK acts in vivo to simplify chromosomal topology as Xer recombination interconverts monomeric and dimeric chromosomes.