S
Stephen J. O'Keefe
Researcher at Merck & Co.
Publications - 40
Citations - 3655
Stephen J. O'Keefe is an academic researcher from Merck & Co.. The author has contributed to research in topics: Calcineurin & Kinase. The author has an hindex of 27, co-authored 40 publications receiving 3575 citations. Previous affiliations of Stephen J. O'Keefe include United States Military Academy.
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Journal ArticleDOI
FK-506- and CsA-sensitive activation of the interleukin-2 promoter by calcineurin.
Stephen J. O'Keefe,Jun'ichi Tamura,Jun'ichi Tamura,Randall L. Kincaid,Michael J. Tocci,Edward A. O'Neill +5 more
TL;DR: It is reported that transfection of a calcineurin catalytic subunit increases the 50% inhibitory concentration of the immunosuppressants FK-506 and CsA, and that a mutant subunit acts in synergy with phorbol ester alone to activate the interleukin-2 promoter in a drug-sensitive manner.
Journal ArticleDOI
Calcineurin acts in synergy with PMA to inactivate I kappa B/MAD3, an inhibitor of NF-kappa B.
Betsy Frantz,E.C. Nordby,G D Bren,N. Steffan,C.V. Paya,R.L. Kincaid,Michael J. Tocci,Stephen J. O'Keefe,Edward A. O'Neill +8 more
TL;DR: These data provide the first demonstration in vivo thatactivation of a protein phosphatase can inactivate I kappa B, and suggest one possible explanation for mechanism‐based toxicities associated with FK‐506 and CsA by demonstrating that these drugs can inhibit the calcineurin‐dependent activation of a virtually ubiquitous transcription factor.
Journal ArticleDOI
Design and synthesis of potent, selective, and orally bioavailable tetrasubstituted imidazole inhibitors of p38 mitogen-activated protein kinase.
Nigel J. Liverton,John W. Butcher,Christopher F. Claiborne,David A. Claremon,Brian E. Libby,Kevin Nguyen,Steven M. Pitzenberger,H. G. Selnick,Garry R. Smith,Andrew J. Tebben,Joe P. Vacca,Sandor L. Varga,Agarwal L,K B Dancheck,A J Forsyth,D S Fletcher,B. Frantz,W A Hanlon,C F Harper,S J Hofsess,M Kostura,Jiunn H. Lin,S Luell,Edward A. O'Neill,Stephen J. O'Keefe +24 more
TL;DR: 48, a potent and selective p38 MAP kinase inhibitor which inhibits lipopolysaccharide-stimulated release of TNF-alpha from human blood with an IC50 2.2 nM shows good oral bioavailability in rat and rhesus monkey, and demonstrates significant improvement in measures of disease progression in a rat adjuvant-induced arthritis model.
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The activation state of p38 mitogen-activated protein kinase determines the efficiency of ATP competition for pyridinylimidazole inhibitor binding.
Betsy Frantz,Tracey Klatt,Margaret Pang,Janey N. Parsons,Anna M. Rolando,Hollis R. Williams,Michael J. Tocci,Stephen J. O'Keefe,Edward A. O'Neill +8 more
TL;DR: It is demonstrated in vivo that at concentrations consistent with its IC50 as a cytokine inhibitor, SB203580 can inhibit stimulus-induced phosphorylation of p38 at the Thr-Gly-Tyr activation motif.
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Structural basis for p38alpha MAP kinase quinazolinone and pyridol-pyrimidine inhibitor specificity.
Catherine E. Fitzgerald,Sangita B. Patel,Joseph W. Becker,Patricia M. Cameron,Dennis M. Zaller,Vasilis Bill Pikounis,Stephen J. O'Keefe,Giovanna Scapin +7 more
TL;DR: Comparing the crystal structures of p38 bound to four different compounds shows that binding of the more specific molecules is characterized by a peptide flip between Met109 and Gly110, which confirms that the selectivity of quinazolinones and pyridol-pyrimidines results from the presence of a glycine in position 110.