Stephen Oroszlan

TL;DR: In this paper, a protein preparation was judged to be homogeneous IL 3 by the following criteria: 1) elution of a peak of IL 3 with a constant specific activity in the last step of purification, 2) presence of a single protein by SDS-PAGE analysis, 3) receptor-binding activity against IL 3-dependent cell lines, 4) a specific activity of congruent to 0.2 ng/ml required for 50% of maximal biologic activity, and 5) the presence of an amino terminal sequence.

TL;DR: Approximately 80 percent of all human sera that react with antigens of HTLV-III, the etiologic agent of the acquired immune deficiency syndrome (AIDS), recognize protein bands at 66 and 51 kilodaltons.

TL;DR: Radiolabeled amino acid sequencing was used to characterize gp41, an antigen of HTLV-III/LAV, the virus believed to be the etiological agent of the acquired immune deficiency syndrome, and placed gp41 in the COOH-terminal region of the env gene product.

TL;DR: The techniques in this study can be used to define the extent of variability of the env gene product among different virus isolates and may identify the nature and patterns of the humoral immune response that lead to an immunologically protected state.

TL;DR: Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 50–100-fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques.