Showing papers in "Analytical Biochemistry in 1973"

TL;DR: A new method for determination of uronic acids with meta-hydroxydiphenyl is introduced, which is simpler, quicker, more sensitive, and more specific than other methods, and it needs lesser amounts of fluid.

TL;DR: This protein assay is described in which the sample is precipitated with trichloroacetic acid in the presence of sodium dodecylsulfate, filtered off on a Millipore membrane and stained with Amidoschwarz 10B, and its absorbance determined at 630 nm.

TL;DR: A simple procedure is described for removal of Triton X-100 from protein samples by adsorption of the detergent on a commercial copolymer in bead form with no loss or dilution of protein.

TL;DR: The plasma norepinephrine and epinephrine assay, when compared to currently available methods, provides a substantial decrease in the assay time while providing a 10-fold increase in sensitivity which allows the analysis to be performed on 0.75 ml or less of plasma.

TL;DR: The dissociation of the soluble apoproteins from lipid by TMU obviates the need for time-consuming delipidation by organic solvents, permitting immediate electrophoretic analysis in polyacrylamide gels.

TL;DR: Equations are presented for the analysis of ternary mixtures of these hemoglobin derivatives as well as those of ferrihemoglobin between pH 6.2 and 8.8.

TL;DR: A scintillation cocktail containing 25% Triton X-114 in xylene is considered for a broad range of scintsillation counting applications and gives good counting efficiencies for 3H and 14C.

TL;DR: An improved PAS method for the detection of glycoproteins after electrophoresis on acrylamid or urea-acrylamide gels is described, with stronger oxidizing conditions and a more controlled washing for the removal of periodic acid resulting in both increased staining intensity and band resolution.

TL;DR: Detergent-solubilized acetylcholine receptors from the electric organs of Electrophorus electricus and Torpedo californica are rapidly, quantitatively and specifically assayed by exposure to 125I-α-bungarotoxin and subsequent adsorption onto DEAE-cellulose paper disks.

TL;DR: A new cycling assay for NAD that uses thiazolyl blue as a terminal electron acceptor has been found to offer significant advantages over the more established procedure that employs 2,6-dichlorophenolindophenol.

TL;DR: A sensitive fluorometric assay for measurement of lipid peroxidation damage to biological preparations and tissues is described and the amount of lipid-soluble fluorescent material in extracted samples was directly proportional to that added before extraction.

TL;DR: A new simple colorimetric assay for measurement of peroxidase activity using 3,3′-diaminobenzidine tetrahydrochloride as hydrogen donor is described, and the DAB method appears more sensitive than the o-dianisidine and the guaiacol assays for per oxidase.

TL;DR: Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 50–100-fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques.

TL;DR: The method is based on the direct fluorimetric measurement of the metabolites of benzo[α]pyrene in alkaline medium after adding Triton X 100 to the testing sample.

TL;DR: The method has a number of advantages compared to those previously described; the cycling rate is high; blank values are low; the reaction is linear over a wide range of NAD concentrations; and the terminal indicator reaction requires only one step.

TL;DR: The method is applicable to measurement of flavin concentrations in the nanomolar range and can be readily used for determination of the flavin composition of purified flavoproteins.

TL;DR: The concentration of proteins in solutions or cell-free extracts contaminated by tRNA's or rRNA's can be simply, rapidly, and accurately determined by measuring the absorbance at 2285 and 2345 A, two wavelengths where the absorption due to ribonucleic acids is identical so that their interference can be eliminated.

TL;DR: A rapid and precise method for the determination of brain 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) activity has been developed and could be completed in less than 1 hr.

TL;DR: A method is described for the simultaneous analysis of endogenous and d 4 -labeled tracer variants of choline and acetylcholine by a gas chromatograph/mass spectrometer system operated in the specific ion detection mode.

TL;DR: Human serum proteins were analyzed by crossed immuno-affinoelectrophoresis and affinity chromatography on concanavalin A bound to Sepharose and concordant results were obtained.

TL;DR: The findings suggest that binding of purified proteins for agarose substituted with 4-phenylbutylamine or with ϵ-aminocaproyl- d -tryptophan methyl ester results from the combined (and possibly mutually reinforcing) effects of hydrophobic and electrostatic forces.

TL;DR: A comparison of the direct spectrophotometric estimation of ferrocyanide with a modified folin-Wu method for estimation of blood glucose should indicate the possibility of its application to biological fluids containing cells and proteins.

TL;DR: The product of this preparative method has been examined for the presence of minor hemoglobin components, ferrihemoglobin, and phosphates, and its behavior was found to be consistent with that observed by other investigators for unfractionated, phosphate-free hemoglobin.

TL;DR: A sensitive method is reported to localize cystine-containing peptides in column effluents through reduction of disulfide bonds by sodium borohydride in guanidine followed by destruction of excess of sodium borbohydride by acid and acetone.

TL;DR: A method for staining the phosphate moiety of phospho-proteins after electrophoresis on polyacrylamide gels using methyl green dissolved in dilute acetic acid and one nanomole of protein-bound phosphate is detectable.

TL;DR: Capable of assaying as little as 40 μg of Triton X-100, the procedure is applicable in the presence of proteins provided a small easily determined correction is applied.