S
Steven J. Clough
Researcher at University of Illinois at Urbana–Champaign
Publications - 61
Citations - 23314
Steven J. Clough is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Gene & Gene expression. The author has an hindex of 30, co-authored 56 publications receiving 21506 citations. Previous affiliations of Steven J. Clough include Agricultural Research Service & United States Department of Agriculture.
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Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
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The Arabidopsis dnd1 “defense, no death” gene encodes a mutated cyclic nucleotide-gated ion channel
Steven J. Clough,Kevin Fengler,Kevin Fengler,I-ching Yu,I-ching Yu,Bernadette Lippok,Bernadette Lippok,Roger Smith,Roger Smith,Andrew F. Bent,Andrew F. Bent +10 more
TL;DR: It is found that salicylic acid is required for the elevated resistance caused by the dnd1 mutation but that removal of salicyric acid did not completely eliminate the dwarf and loss-of-HR phenotypes of mutant dND1 plants.
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Biotic stress globally downregulates photosynthesis genes.
Damla D. Bilgin,Jorge A. Zavala,Jin Zhu,Steven J. Clough,Steven J. Clough,Donald R. Ort,Evan H. DeLucia +6 more
TL;DR: It is hypothesized that slow turnover of many photosynthesis-related proteins allows plants to invest resources in immediate defence needs without debilitating near term losses in photosynthetic capacity.
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Female Reproductive Tissues Are the Primary Target of Agrobacterium-Mediated Transformation by the Arabidopsis Floral-Dip Method
TL;DR: The results suggest that ovules are the site of productive transformation in the floral-dip method, and suggest that Agrobacterium must be delivered to the interior of the developing gynoecium prior to locule closure if efficient transformation is to be achieved.
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Identification of Four Soybean Reference Genes for Gene Expression Normalization
Marc Libault,Sandra Thibivilliers,Damla D. Bilgin,Osman Radwan,Marisol Benitez,Steven J. Clough,Gary Stacey +6 more
TL;DR: Four soybean genes are confirmed as new reference genes (annotated as ATP‐binding cassette [ABC] transporter, F‐box protein family, metalloprotease, and CDPK‐related protein kinase) that should be useful for normalization of gene expression studies in soybean, an important crop plant.