Steven J. Wu

TL;DR: Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components, is described.

TL;DR: Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components, is described and demonstrated by profiling histone modifications, RNA Polymerase II and transcription factors on low cell numbers and single cells.

TL;DR: In this article, the CUT&Tag method was adapted to scalable nanowell and droplet-based single-cell platforms to profile chromatin landscapes in single cells from complex tissues and during the differentiation of human embryonic stem cells.

TL;DR: The easy, cost-effective workflow makes automated CUT&RUN an attractive tool for high-throughput characterization of cell types and patient samples, and a continuous metric to identify cell-type-specific promoter and enhancer activities is developed.

TL;DR: In this paper, a subset of KMT2A oncofusion-binding sites are marked by bivalent (H3K4me3 and H3K27me3) chromatin signatures, and single-cell CUT&Tag profiling reveals that these sites display cell-to-cell heterogeneity suggestive of lineage plasticity.