Improvement of enzyme activity, stability and selectivity via immobilization techniques

This article describes the process of formation of droplets and bubbles in microfluidic T-junction geometries. At low capillary numbers break-up is not dominated by shear stresses: experimental results support the assertion that the dominant contribution to the dynamics of break-up arises from the pressure drop across the emerging droplet or bubble. This pressure drop results from the high resistance to flow of the continuous (carrier) fluid in the thin films that separate the droplet from the walls of the microchannel when the droplet fills almost the entire cross-section of the channel. A simple scaling relation, based on this assertion, predicts the size of droplets and bubbles produced in the T-junctions over a range of rates of flow of the two immiscible phases, the viscosity of the continuous phase, the interfacial tension, and the geometrical dimensions of the device.

http://ichf.pong.pl/i/publications/29.pdf

Formation of droplets and bubbles in a microfluidic T-junction-scaling and mechanism of break-up.

Biocompatibility, Pharmaceutical and Biomedical Applications L. Harivardhan Reddy,†,‡ Jose ́ L. Arias, Julien Nicolas,† and Patrick Couvreur*,† †Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, Universite ́ Paris-Sud XI, UMR CNRS 8612, Faculte ́ de Pharmacie, IFR 141, 5 rue Jean-Baptiste Cleḿent, F-92296 Chat̂enay-Malabry, France Departamento de Farmacia y Tecnología Farmaceútica, Facultad de Farmacia, Campus Universitario de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain ‡Pharmaceutical Sciences Department, Sanofi, 13 Quai Jules Guesdes, F-94403 Vitry-sur-Seine, France

Magnetic nanoparticles: design and characterization, toxicity and biocompatibility, pharmaceutical and biomedical applications.

Enzyme stability and stabilization—Aqueous and non-aqueous environment

Potential applications of enzymes immobilized on/in nano materials: A review

Effects of Pressure and Dissolved Oxygen Concentration on Growth of Pseudomonas aeruginosa : Studies on Fermentation under Increased Pressure (II)

Commercial grade lipases from Rhizopus japonicus, R. delemar, and Rhizomucor miehei were modified by surfactant (sorbitan monostearate), and their protein recovery and interesterification, and hydrolysis activities were investigated. By repeating the lipase modification processes three times, total protein recoveries of 17–35% could be obtained. The original lipases had no interesterification activities at all; however, all modified lipases in the first process had significant interesterification activities. In the hydrolysis reactions, all modified lipases obtained from the first process showed about three times higher specific activities than the original lipases. The modified lipases obtained from the second and third processes had lower specific interesterification and hydrolysis activities than the lipases from the first one. These results suggest that the surfactant modification process is effective not only for interesterification but also lipase purification.

Sukekuni Mukataka

Papers

Effects of Pressure and Dissolved Oxygen Concentration on Growth of Pseudomonas aeruginosa : Studies on Fermentation under Increased Pressure (II)

Surfactant modification of lipases for lipid interesterification and hydrolysis reactions

Preparation of Giant Vesicles Larger than 30 μm That Entrap a Model Hydrophilic Substance Using a Size-controlled Water-in-Oil Emulsion

Effects of Axial Dispersion and Mass Transfer Resistance on Conversion in a Hollow Fiber Enzyme Reactor

Effect of substrate presoaking treatment of support materials on the activity of immobilized glucoamylase.