Sumeer Razdan

TL;DR: Quantitative RT-PCR analysis showed that squalene synthase (WsSQS) expressed in all tested tissues including roots, stem and leaves with the highest level of expression in leaves, and up-regulated by different signalling components including methyl-jasmonate, salicylic acid and 2, 4-D, which was consistent with the predicted results of WsS QS promoter region.

TL;DR: Members of OSC superfamily from Withania somnifera, a medicinal plant of immense repute known to synthesize a large array of biologically active steroidal lactone triterpenoids called withanolides, were characterized and negative regulator channelizes substrate pool toward cycloartenol synthase at subdividing junction leading to enhanced withanolide production.

TL;DR: A detailed overview of various studies undertaken by the authors and collated literature related to molecular and in vitro approaches employed in W. somnifera for understanding various molecular network interactions in entirety is provided.

TL;DR: High performance liquid chromatography of withanolides extracted from elicitor-treated samples showed a significant increase in two of the key withanolide A and withaferin A, possibly indicating the role of WsCPR2 in withanolid biosynthesis.

TL;DR: This is the first report of cloning and bacterial expression of squalene epoxidase (SE) gene from W. somnifera and may be of significant interest to understand the regulatory role of SE in the biosynthesis of withanolides.