S
Sylvain W. Lapan
Researcher at Harvard University
Publications - 27
Citations - 2897
Sylvain W. Lapan is an academic researcher from Harvard University. The author has contributed to research in topics: Planarian & Schmidtea mediterranea. The author has an hindex of 17, co-authored 26 publications receiving 2268 citations. Previous affiliations of Sylvain W. Lapan include Howard Hughes Medical Institute & Massachusetts Institute of Technology.
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Journal ArticleDOI
Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics.
Karthik Shekhar,Sylvain W. Lapan,Sylvain W. Lapan,Irene E. Whitney,Nicholas M. Tran,Evan Z. Macosko,Evan Z. Macosko,Monika S. Kowalczyk,Xian Adiconis,Joshua Z. Levin,James Nemesh,James Nemesh,Melissa Goldman,Melissa Goldman,Steven A. McCarroll,Steven A. McCarroll,Constance L. Cepko,Constance L. Cepko,Aviv Regev,Aviv Regev,Aviv Regev,Joshua R. Sanes +21 more
TL;DR: This work provides a systematic methodology for achieving comprehensive molecular classification of neurons, identifies novel neuronal types, and uncovers transcriptional differences that distinguish types within a class.
Journal ArticleDOI
Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues.
Sinem K. Saka,Yu Wang,Yu Wang,Jocelyn Y. Kishi,Jocelyn Y. Kishi,Allen Zhu,Allen Zhu,Yitian Zeng,Yitian Zeng,Wenxin Xie,Wenxin Xie,Koray Kirli,Clarence Yapp,Marcelo Cicconet,Brian J. Beliveau,Brian J. Beliveau,Brian J. Beliveau,Sylvain W. Lapan,Siyuan Yin,Siyuan Yin,Millicent Lin,Millicent Lin,Edward S. Boyden,Pascal S. Kaeser,German Pihan,George M. Church,George M. Church,Peng Yin,Peng Yin +28 more
TL;DR: Immuno-SABER achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction, which presents an effective and accessible platform for multiplexing and amplified imaging of proteins with high sensitivity and throughput.
Journal ArticleDOI
SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues.
Jocelyn Y. Kishi,Sylvain W. Lapan,Brian J. Beliveau,Brian J. Beliveau,Brian J. Beliveau,Emma R. West,Allen Zhu,Allen Zhu,Hiroshi Sasaki,Hiroshi Sasaki,Sinem K. Saka,Sinem K. Saka,Yu Wang,Yu Wang,Constance L. Cepko,Constance L. Cepko,Peng Yin,Peng Yin +17 more
TL;DR: This work introduces signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands, resulting in up to 450-fold signal amplification.
Journal ArticleDOI
A molecular wound response program associated with regeneration initiation in planarians
TL;DR: Most planarian wound-induced genes are conserved across metazoans, and identified genes and mechanisms should be important broadly for understanding wound signaling and regeneration initiation.
Journal ArticleDOI
Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea
TL;DR: It is suggested that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblast subsets.