E
Evan Z. Macosko
Researcher at Broad Institute
Publications - 90
Citations - 21758
Evan Z. Macosko is an academic researcher from Broad Institute. The author has contributed to research in topics: Biology & Medicine. The author has an hindex of 28, co-authored 63 publications receiving 15165 citations. Previous affiliations of Evan Z. Macosko include Harvard University & Howard Hughes Medical Institute.
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Journal ArticleDOI
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
Evan Z. Macosko,Evan Z. Macosko,Anindita Basu,Anindita Basu,Rahul Satija,Rahul Satija,James Nemesh,James Nemesh,Karthik Shekhar,Melissa Goldman,Melissa Goldman,Itay Tirosh,Allison R. Bialas,Nolan Kamitaki,Nolan Kamitaki,Emily M. Martersteck,John J. Trombetta,David A. Weitz,Joshua R. Sanes,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Aviv Regev,Aviv Regev,Aviv Regev,Steven A. McCarroll,Steven A. McCarroll +26 more
TL;DR: Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together.
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
Evan Z. Macosko,Evan Z. Macosko,Anindita Basu,Anindita Basu,Rahul Satija,Rahul Satija,James Nemesh,James Nemesh,Karthik Shekhar,Melissa Goldman,Melissa Goldman,Itay Tirosh,Allison R. Bialas,Nolan Kamitaki,Nolan Kamitaki,Emily M. Martersteck,John J. Trombetta,David A. Weitz,Joshua R. Sanes,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Aviv Regev,Aviv Regev,Aviv Regev,Steven A. McCarroll,Steven A. McCarroll +26 more
TL;DR: Drop-seq as discussed by the authors analyzes mRNA transcripts from thousands of individual cells simultaneously while remembering transcripts' cell of origin, and identifies 39 transcriptionally distinct cell populations, creating a molecular atlas of gene expression for known retinal cell classes and novel candidate cell subtypes.
Journal ArticleDOI
Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution
Samuel G. Rodriques,Samuel G. Rodriques,Robert R. Stickels,Robert R. Stickels,Aleksandrina Goeva,Carly A. Martin,Evan Murray,Charles R. Vanderburg,Joshua D. Welch,Linlin M. Chen,Fei Chen,Evan Z. Macosko,Evan Z. Macosko +12 more
TL;DR: Slide-seq provides a scalable method for obtaining spatially resolved gene expression data at resolutions comparable to the sizes of individual cells, and defines the temporal evolution of cell type–specific responses in a mouse model of traumatic brain injury.
Journal ArticleDOI
Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes.
Timothy R. Hammond,Timothy R. Hammond,Timothy R. Hammond,Connor Dufort,Lasse Dissing-Olesen,Lasse Dissing-Olesen,Lasse Dissing-Olesen,Stefanie Giera,Stefanie Giera,Adam Young,Alec Wysoker,Alec J. Walker,Alec J. Walker,Alec J. Walker,Frederick Gergits,Michael Segel,James Nemesh,Samuel E. Marsh,Samuel E. Marsh,Samuel E. Marsh,Arpiar Saunders,Arpiar Saunders,Evan Z. Macosko,Florent Ginhoux,Jinmiao Chen,Robin J.M. Franklin,Xianhua Piao,Xianhua Piao,Steven A. McCarroll,Steven A. McCarroll,Beth Stevens +30 more
TL;DR: The analysis of RNA expression patterns of more than 76,000 individual microglia in mice during development, in old age, and after brain injury uncovered at least nine transcriptionally distinct microglial states, which expressed unique sets of genes and were localized in the brain using specific markers.
Journal ArticleDOI
Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain.
Arpiar Saunders,Arpiar Saunders,Evan Z. Macosko,Evan Z. Macosko,Alec Wysoker,Alec Wysoker,Melissa Goldman,Melissa Goldman,Fenna M. Krienen,Fenna M. Krienen,Heather de Rivera,Heather de Rivera,Elizabeth Bien,Elizabeth Bien,Matthew A. Baum,Matthew A. Baum,Laura Bortolin,Laura Bortolin,Shuyu Wang,Aleksandrina Goeva,James Nemesh,James Nemesh,Nolan Kamitaki,Nolan Kamitaki,Sara A. Brumbaugh,Sara A. Brumbaugh,David Kulp,David Kulp,Steven A. McCarroll,Steven A. McCarroll +29 more
TL;DR: Features of brain organization are revealed, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions.