Tetsuya Kimura

TL;DR: A new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone.

TL;DR: Improved pGWBs should be a powerful tool for transgenic research in plants because of their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing.

TL;DR: The Structure of Cellulases and Their Applications as discussed by the authors was the first paper to describe the structure of cellulases in terms of their applications in biology and genetic engineering, and was published in 1997.

TL;DR: N-terminal amino acid sequence analysis of the C. josui cellulosomal proteins indicates that both CipA and CelD are major components of the cellulosome.

TL;DR: A new series of Gateway binary vectors that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA, and have a great advantage in expressing a cDNA under the regulation of desired promoters.