M
Makoto Kawamukai
Researcher at Shimane University
Publications - 151
Citations - 6216
Makoto Kawamukai is an academic researcher from Shimane University. The author has contributed to research in topics: Schizosaccharomyces pombe & Mutant. The author has an hindex of 38, co-authored 142 publications receiving 5691 citations.
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Journal ArticleDOI
Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
Tsuyoshi Nakagawa,Takayuki Kurose,Takeshi Hino,Katsunori Tanaka,Makoto Kawamukai,Yasuo Niwa,Kiminori Toyooka,Ken Matsuoka,Tetsuro Jinbo,Tetsuya Kimura +9 more
TL;DR: A new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone.
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Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation.
Katsunori Tanaka,Junko Nishide,Koei Okazaki,Hiroaki Kato,Osami Niwa,Tsuyoshi Nakagawa,Hideyuki Matsuda,Makoto Kawamukai,Yota Murakami +8 more
TL;DR: The identification of the SUMO-1 homologue of fission yeast, which is shown to be required for a number of nuclear events including the control of telomere length and chromosome segregation, suggests that several nuclear proteins are regulated by Pmt3p conjugation.
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PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae
TL;DR: Using gene disruptants, transformants and cell-free extracts of these strains, it is verified that the adjacent PAD1 and FDC1 genes are essential for the decarboxylation of phenylacrylic acids in S. cerevisiae.
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Biosynthesis, bioproduction and novel roles of ubiquinone.
TL;DR: Recent findings relating to the biosynthesis, bioproduction and novel roles of ubiquinone are summarized and a role as a lipid soluble antioxidant is now widely accepted.
Journal ArticleDOI
Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
Tsuyoshi Nakagawa,Shinya Nakamura,Katsunori Tanaka,Makoto Kawamukai,Takamasa Suzuki,Kenzo Nakamura,Tetsuya Kimura,Sumie Ishiguro +7 more
TL;DR: A new series of Gateway binary vectors that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA, and have a great advantage in expressing a cDNA under the regulation of desired promoters.