T
Thomas E. Spratt
Researcher at Pennsylvania State University
Publications - 68
Citations - 1927
Thomas E. Spratt is an academic researcher from Pennsylvania State University. The author has contributed to research in topics: DNA polymerase & DNA. The author has an hindex of 26, co-authored 66 publications receiving 1801 citations. Previous affiliations of Thomas E. Spratt include Ohio State University & Penn State Cancer Institute.
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Journal ArticleDOI
Interaction of Sirt3 with OGG1 contributes to repair of mitochondrial DNA and protects from apoptotic cell death under oxidative stress
Yan Cheng,X. Ren,A. S.P. Gowda,Y. Shan,Li Zhang,Y. S. Yuan,R. Patel,H. Wu,Kathryn J. Huber-Keener,J. W. Yang,David Liu,Thomas E. Spratt,Jin Ming Yang +12 more
TL;DR: A new function and mechanism is revealed for Sirt3 in defending the mitochondrial genome against oxidative damage and protecting from the genotoxic stress-induced apoptotic cell death but also provides evidence supporting a new mtDNA repair pathway.
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Evidence for 4-(3-pyridyl)-4-oxobutylation of DNA in F344 rats treated with the tobacco-specific nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N'-nitrosonornicotine.
TL;DR: Results demonstrate that 4-(3-pyridyl)-4-oxobutylation of DNA occurs in rats treated with NNK or NNN, and are consistent with the hypothesis that these nitrosamines are metabolically activated by alpha-hydroxylation.
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Pyridyloxobutyl adduct O6-[4-oxo-4-(3-pyridyl)butyl]guanine is present in 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone-treated DNA and is a substrate for O6-alkylguanine-DNA alkyltransferase.
Lijuan Wang,Thomas E. Spratt,Xiao-Keng Liu,†,‖,Stephen S. Hecht,and Anthony E. Pegg,Lisa A. Peterson +5 more
TL;DR: O6-pobG was removed, presumably a result of transfer of the pyridyloxobutyl group from the O6-position of guanine to AGT's active site, when [5-3H]NNKOAc-treated DNA was incubated with either rat liver or recombinant human AGT.
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An unprecedented nucleic acid capture mechanism for excision of DNA damage
TL;DR: Crystal structures of the recently discovered Bacillus cereus AlkD glycosylase in complex with DNAs containing alkylated, mismatched and abasic nucleotides are presented, providing an illustration for how hydrolysis of N3- and N7-alkylated bases may be facilitated by increased lifetime out of the DNA helix.
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Glucuronidation of tobacco-specific nitrosamines by UGT2B10.
TL;DR: UGT2B10 is likely the most active UGT isoform in human liver for the N-glucuronidation of TSNAs, and real-time polymerase chain reaction analysis showed that it was expressed at a level that was 26% higher than that observed for UGT1A4 in a screening of normal liver tissue specimens from 20 individual subjects.