Thomas E. Wilson

TL;DR: How double-strand break structure, the yeast NHEJ proteins, and alternative rejoining mechanisms influence the accuracy of break repair is reviewed and how the balance between N HEJ and homologous repair is regulated by cell state to promote genome preservation is considered.

TL;DR: There is great interest in defining alt-N HEJ more precisely, including its regulation relative to c-NHEJ, in light of evidence that alt- NHEJ can execute chromosome rearrangements.

TL;DR: It is found that genes with rapid elongation rates showed higher densities of H3K79me2 and H4K20me1 histone marks compared to slower elongating genes, and features that negatively correlated with elongation rate included the density of exons, long terminal repeats, GC content of the gene, and DNA methylation density in the bodies of genes.

TL;DR: It is demonstrated that prokaryotic Ku and ligase form a bona fide NHEJ system that encodes all the recognition, processing, and ligation activities required for DSB repair.

TL;DR: It is reported that the tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae and it is shown that yeast lacking the Rad1/Rad10-related proteins Mus81-Mms4 display a unique pattern of camptothecin sensitivity and suggest a concerted model for the action of these endonucleases.