Showing papers by "Thomas W. Wakefield published in 1995"
TL;DR: In a rat model of inferior vena caval thrombosis, vein wall was temporally examined for inflammation by assessment of histopathology, leukocyte morphometrics, and cytokine levels as discussed by the authors.
Abstract: Thrombosis and inflammation are closely related. However, the response of the vein wall to venous thrombosis has been poorly documented. This study examines the hypothesis that venous thrombosis is associated with an inflammatory response in the vein wall. In a rat model of inferior vena caval thrombosis, vein wall was temporally examined for inflammation by assessment of histopathology, leukocyte morphometrics, and cytokine levels. Animals were killed 1 hour and 1, 3, and 6 days after thrombus induction. Our findings demonstrated an early (day 1) neutrophil infiltration into the vein wall followed by a later (days 3 and 6) monocyte/macrophage and lymphocyte response. Cytokines were elevated only under conditions of venous thrombosis. Levels of epithelial neutrophil activating protein–78 (ENA-78), tumor necrosis factor–α (TNF), interleukin-6, and JE/monocyte chemoattractant protein–1 (JE/MCP-1) increased over the 6-day period, while macrophage inflammatory protein-1α (MIP-1α) peaked at day 3 after thrombus induction. Additionally, rats were passively immunized with neutralizing antibodies to TNF, ENA-78, MIP-1α, JE/MCP-1, intercellular adhesion molecule–1 (ICAM-1), and CD18 compared with control antibodies. The most effective antibody early after thrombus induction for attenuating vein wall neutrophil extravasation was anti-TNF ( P <.01). The monocyte/macrophage extravasation was inhibited most by anti–ICAM-1 followed by anti-TNF ( P <.01). These findings demonstrate that venous thrombosis is associated with significant vein wall inflammation that is partially inhibited by neutralizing antibodies to cytokines and adhesion molecules.
171 citations
TL;DR: G gadolinium-enhanced MRA of AAA provides appropriate, essential anatomic information for aortic reconstructive surgery in a 1-hour examination devoid of contrast-related renal toxicity or catheterization-related complications attending conventional arteriography.
130 citations
TL;DR: Pediatric patients with renovascular hypertension clearly benefit from carefully executed operative therapy, and contemporary surgical management emphasizes direct reimplantation of main renal arteries into the aorta, reim Plantation of segmental arteries into adjacent renal arteries, patch aortoplasty for associated abdominal aortic coarctations, and single-stage revascularizations.
116 citations
Journal Article•
TL;DR: It is demonstrated that venous thrombosis is associated with significant vein wall inflammation that is partially inhibited by neutralizing antibodies to cytokines and adhesion molecules.
49 citations
TL;DR: The unique findings from the present study suggest that a fundamental contributory mechanisms for the changes in LV function observed after protamine administration may be the direct effect of unbound protamine on myocyte contractile processes.
Abstract: Background Administration of the arginine-rich, highly charged protamine (PROT) molecule has been associated with episodes of acute left ventricular (LV) dysfunction. The objective of the present study was to test the hypothesis that PROT has direct effects on isolated LV myocyte contractile processes and sarcolemmal transduction systems. Methods and Results Exposure of porcine LV myocytes (n=305) to 40 μg/mL PROT (reflecting a dose of 2.5 mg/kg) decreased basal contractile function and β-adrenergic responsiveness. For example, myocyte percent shortening was 4.3±0.1% in control myocytes and decreased to 2.8±0.2% in the presence of 40 μg/mL PROT (P 40% from forskolin. In addition, PROT abolished the inotro...
28 citations
TL;DR: Important world-wide differences exist regarding prophylaxis for postoperative graft occlusion, andconsiderable geographical differences regarding patient selection, the frequency of specific procedures and operative techniques existed.
18 citations
TL;DR: This investigation documents that a new designer variant of protamine [+18BE] has superior efficacy compared with [+21] standard protamine for reversal of LMWH anticoagulation and that this occurs with a highly favorable toxicity profile.
16 citations
TL;DR: A case of temporary facial nerve palsy during a carotid endarterectomy performed under regional anesthesia supplemented with local anesthetic after inadequate cervical plexus blockade is reported.
Abstract: T he cervical plexus block can be advantageous for carotid endarterectomy, allowing the anesthesiologist and surgeon to monitor the neurologic status of the patient. On occasion, inadequate blockade necessitates a change of technique to either local infiltration or general anesthesia. We report a case of temporary facial nerve palsy during a carotid endarterectomy performed under regional anesthesia supplemented with local anesthetic after inadequate cervical plexus blockade.
11 citations