Showing papers by "Timothy L. Ratliff published in 2008"
TL;DR: The findings demonstrate for the first time that platelet-derived membrane vesicles are sufficient to deliver CD154 to stimulate antigen-specific IgG production and modulate germinal center formation through cooperation with responses elicited by CD4(+) T cells.
236 citations
TL;DR: In a physiologic setting that mimics wounding, platelets perform a sentinel function when antigen dose is too low to provoke an efficient immune response, and can enhance the generation of antigen-specific CD8 T cells that are functionally relevant to the host.
86 citations
TL;DR: The data demonstrating a correlation between inflammation and prostate cancer is reviewed as well as what is currently known about the inflammatory response in the prostate and the impact this inflammation has on the prostate tissue.
Abstract: Recent studies have identified a role for inflammation in the development and progression of several cancers, such as liver, stomach and the large intestine. Data from several studies has shown correlations between soluble inflammatory mediators, such as cytokines, chemokines and growth factors. However, a direct relationship between inflammation and prostate cancer has yet to be identified. Two major hurdles currently exist which limit the study of this relationship are first that animal models available for studying prostate inflammation are limited, and secondly that relatively little is known about the inflammatory response in the prostate. Here we first review the data demonstrating a correlation between inflammation and prostate cancer as well as review what is currently known about the inflammatory response in the prostate and the impact this inflammation has on the prostate tissue.
82 citations
Journal Article•
TL;DR: It is shown in this journal that platelets modulate innate and adaptive immunity and soluble CD40 ligand derived and cleaved from platelets is responsible for acute transfusion reactions (ATR).
52 citations
TL;DR: It is found that CD154 is abundantly expressed in platelet precursor cells, megakaryocytes, and this work suggests that platelet-associated CD154 can be biochemically modulated.
36 citations
TL;DR: FAP is an effective antitumor agent that inhibits tumor growth at a level equivalent to that observed for BCG, and may provide an alternative to BCG for treatment of superficial bladder cancer.
Abstract: Purpose
The receptor responsible for the attachment of bacillus Calmette-Guerin (BCG) to fibronectin, fibronectin attachment protein (FAP), has been cloned. Studies targeting FAP as an inducer of immunity in mycobacterial infections suggest that FAP is a highly immunogenic protein. In light of these findings and the need to find effective alternatives to BCG treatment for bladder cancer, we tested the ability of FAP to induce antitumor activity.
27 citations
TL;DR: Findings suggest ON causes greater immune suppression than LN in the porcine model, and produces higher CRP concentrations postoperatively, a larger decrease in T-cell proliferation ability, and more IL-10 activity than Ln or sham treatment.
Abstract: Purpose: To determine differences in the systemic and cell-specific immune response to open and laparoscopic nephrectomy in the porcine model. Materials and Methods: Twenty male pigs (25–40 kg) were vaccinated with human adenovirus containing ovalbumin (Ova) and 3 weeks later underwent a sham procedure (N = 4), laparoscopic nephrectomy (LN)(N = 8), or open nephrectomy (ON) (N = 8). Blood was collected after anesthesia induction and immediately and 24 and 48 hours postoperatively and assayed for complete blood count (CBC), cortisol, and C-reactive protein (CRP). Natural killer (NK) cells were isolated and stimulated in vitro for 48 hours with polyinosinic:polycytidylic acid (Poly I:C) and interleukin (IL)-2 to determine cytotoxic activity. Peripheral blood mononuclear cells (PBMC) were isolated for flow cytometry staining with CD8, CD4, and CD25 markers. Additional PBMCs were stimulated in vitro with Ova and ConA for 48 hours to measure the production of IL-10 and interferon (IFN)-γ and a thymidine-incorpo...
14 citations
4 citations
TL;DR: In vivo animal data reporting biologic platelet CD154 function coupled with reports of direct biologic activity on isolated primary human neutrophils and B cells indicate plateletCD154 could have clinically significant function.
1 citations