T
Tjibbe Bosma
Researcher at MorphoSys
Publications - 24
Citations - 1236
Tjibbe Bosma is an academic researcher from MorphoSys. The author has contributed to research in topics: Haloalkane dehalogenase & Phage display. The author has an hindex of 16, co-authored 24 publications receiving 1138 citations. Previous affiliations of Tjibbe Bosma include University of Groningen.
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Journal ArticleDOI
Halohydrin Dehalogenases Are Structurally and Mechanistically Related to Short-Chain Dehydrogenases/Reductases
Johan E. T. van Hylckama Vlieg,Lixia Tang,Jeffrey H. Lutje Spelberg,Tim Smilda,Gerrit J. Poelarends,Tjibbe Bosma,Annet E. J. van Merode,Marco W. Fraaije,Dick B. Janssen +8 more
TL;DR: A site-directed mutagenesis study, with HheC as a model enzyme, supports a mechanism for halohydrin dehalogenases in which the conserved Tyr145 acts as a catalytic base and Ser132 is involved in substrate binding, since it does not involve a covalent enzyme-substrate intermediate.
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Novel surface display system for proteins on non-genetically modified gram-positive bacteria.
Tjibbe Bosma,Rolf Kanninga,Jolanda Neef,Sandrine A. L. Audouy,Maarten L. van Roosmalen,Anton Steen,Girbe Buist,Jan Kok,Oscar P. Kuipers,George T. Robillard,Kees Leenhouts +10 more
TL;DR: A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable.
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Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene
TL;DR: The results demonstrated that directed evolution of a key catabolic enzyme and its subsequent recruitment by a suitable host organism can be used for the construction of bacteria for the degradation of a toxic and environmentally recalcitrant chemical.
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Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria
Maarten L. van Roosmalen,Rolf Kanninga,Mohamed El Khattabi,Jolanda Neef,Sandrine A. L. Audouy,Tjibbe Bosma,Anneke Kuipers,Eduard Post,Anton Steen,Jan Kok,Girbe Buist,Oscar P. Kuipers,George T. Robillard,Kees Leenhouts +13 more
TL;DR: A non-living and non-genetically modified gram-positive bacterial delivery particle (GEM) that has built-in adjuvant activity and a high loading capacity for externally added heterologous antigens that are fused to a high affinity binding domain is developed.
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A tandem enzyme reaction to produce optically active halohydrins, epoxides and diols
Jeffrey H. Lutje Spelberg,Johan E. T. van Hylckama Vlieg,Tjibbe Bosma,Richard M. Kellogg,Dick B. Janssen +4 more
TL;DR: The recombinant halohydrin dehalogenase from Agrobacterium radiobacter AD1 was used to obtain enantiomerically pure halohydrins and epoxides by kinetic resolution and the reversible conversion was drawn to completion.