T
Tokuei Takahara
Publications - 7
Citations - 510
Tokuei Takahara is an academic researcher. The author has contributed to research in topics: DNA methylation & Restriction landmark genomic scanning. The author has an hindex of 5, co-authored 6 publications receiving 494 citations.
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Journal ArticleDOI
The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.
Shinji Hirotsune,Tokuei Takahara,Nobuya Sasaki,Kenji Hirose,Atsushi Yoshiki,Tomoo Ohashi,Moriaki Kusakabe,Yasufumi Murakami,Masami Muramatsu,S Watanabe,Kazutaka Nakao,Motoya Katsuki,Yoshihide Hayashizaki +12 more
TL;DR: In situ hybridization reveals that the transcript is detected exclusively in the pioneer neurons which guide neuronal cell migration along the radial array, which offers an explanation for how the reeler mutant phenotype causes a disturbance of the complex architecture of the neuronal network.
Journal ArticleDOI
Dysfunction of the Orleans Reeler Gene Arising from Exon Skipping Due to Transposition of a Full-Length Copy of an Active L1 Sequence into the Skipped Exon
Tokuei Takahara,Tomoya Ohsumi,Junro Kuromitsu,Kazuhiro Shibata,Nobuya Sasaki,Yasushi Okazaki,Hideo Shibata,Shigeo Sato,Atsushi Yoshiki,Moriaki Kusakabe,Masami Muramatsu,Minoru Ueki,Kiyoji Okuda,Yoshihide Hayashizaki +13 more
TL;DR: Interestingly, the skipped exon was inserted by the 7104 bp L1 element which carried the full-length stretch of the mouse L1 sequence, consisting of a 212 bp F-type tandem repeat, open reading frame 1 (ORF1), ORF2, the polyadenylation signal and a poly A stretch.
Journal ArticleDOI
A unique downregulation of h2-calponin gene expression in Down syndrome: a possible attenuation mechanism for fetal survival by methylation at the CpG island in the trisomic chromosome 21.
J Kuromitsu,H. Yamashita,H Kataoka,Tokuei Takahara,Masami Muramatsu,T Sekine,N Okamoto,Yasuhiro Furuichi,Yoshihide Hayashizaki +8 more
TL;DR: In this paper, DNA methylation in the CpG island of the h2-calponin gene was investigated with the DNAs of chromosome 21 isolated from DS patients and their parents.
Journal ArticleDOI
Loss of heterozygosity in chromosomes 1, 5, 7 and 13 in mouse hepatoma detected by systematic genome-wide scanning using RLGS genetic map
Tomoya Ohsumi,Yasushi Okazaki,Hisato Okuizumi,K. Shibata,T. Hanami,Yosuke Mizuno,Tokuei Takahara,Nobuya Sasaki,M. Ueda,Masami Muramatsu,K.A. Kerns,Verne M. Chapman,William A. Held,Yoshihide Hayashizaki +13 more
TL;DR: An RLGS-based scanning system to detect DNA alteration in tumor tissues, using 575 mapped spots/loci in a single gel, applied to search for the LOH of hepatoma from an interspecific F1 hybrid between Mus spretus and C57BL/6 with SV40 early T antigen transgene connected to a mouse major urinary protein enhancer/promoter.
Book ChapterDOI
Protocols for RLGS Gel Production
Yasushi Okazaki,Hisato Okuizumi,Nobuya Sasaki,Shuji Takada,Tokuei Takahara,Yoshihide Hayashizaki +5 more
TL;DR: Protocols for two good representative enzyme combinations, combination 1 (NotI-PvuII-PstI) and combination 2 (PacI-EcoRV-MboI), are shown.