T
Tokuji Ikenaka
Researcher at Osaka University
Publications - 174
Citations - 4954
Tokuji Ikenaka is an academic researcher from Osaka University. The author has contributed to research in topics: Amino acid & Peptide sequence. The author has an hindex of 36, co-authored 174 publications receiving 4893 citations. Previous affiliations of Tokuji Ikenaka include Wako Pure Chemical Industries, Ltd.
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Reexamination of the pyridylamination used for fluorescence labeling of oligosaccharides and its application to glycoproteins.
TL;DR: The pyridylamination reaction of sugar chains from glycoproteins was re-investigated to raise the total yield of p Pyridylamino sugars and the stability of sialyl and fucosyl linkages under the present conditions was also studied.
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Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound.
TL;DR: A potential aldehyde group of an oligosaccharide is combined with 2-aminopyridine by means of the reductive amination with sodium cyanoborohydride giving a fluorescent 2-amines derivative, which can be used for the determination of a degree of polymerization, sequence of sugar units, and the preparation of finger prints.
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Identification of a disaccharide (Xyl-Glc) and a trisaccharide (Xyl2-Glc) O-glycosidically linked to a serine residue in the first epidermal growth factor-like domain of human factors VII and IX and protein Z and bovine protein Z.
Hitoshi Nishimura,Shun Ichiro Kawabata,Walter Kisiel,Sumihiro Hase,Tokuji Ikenaka,Toshifumi Takao,Yasutsugu Shimonishi,Sadaaki Iwanaga +7 more
TL;DR: In relation to the trisaccharide sugar chain previously discovered in bovine factors VII and IX, these findings indicate the existence of a Xyl2-Glc-Ser and aXyl-GlC-Ser structure in the first epidermal growth factor-like domains of human factors VIII and IX and protein Z in addition to that of bovines protein Z.
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Improved Method for Fluorescence Labeling of Sugar Chains with Sialic Acid Residues
TL;DR: An improved procedure for the pyridylamination of sialyl sugar chains is described, and the yield of PA-sugar chains was almost quantitative, and desialyl-s sugar chains was low.
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Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography.
TL;DR: A method for the quantitation of picomole amounts of neutral and amino sugars in glycoconjugates was developed and results were satisfactory, with satisfactory results.