Showing papers in "Biochemical and Biophysical Research Communications in 1978"

TL;DR: The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations and allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.

TL;DR: In this paper, the degradation of denatured globin in reticulocyte lysates is markedly stimulated by ATP, and the system is now resolved into two components, designated fractions I and II, in the order of their elution from DEAE-cellulose.

TL;DR: These observations as well as the possible reaction products of O 2 • that may develop in the time course of an experiment, must be considered when planning or interpreting data from a spin trapping experiment.

TL;DR: A very large isotope effect and a significant amount of epimerization for the hydroxylation of norbornane by cytochrome P-450, suggest an initial hydrogen abstraction to give a carbon radical intermediate.

TL;DR: The depletion of the two amines is followed by a striking decrease in the rate of cell replication in both cell lines, illustrating an essential function for putrescine and spermidine in cell division processes.

TL;DR: A detailed correlation of the amino acid sequence, conformation, and self association of A-I will add further insight into the molecular mechanisms involved in protein-protein and protein-lipid interactions.

TL;DR: It is proposed that the mannan-binding protein is the principle mediating the hepatic uptake of glycoproteins with terminal N-acetylglucosamine and/or mannose residues which are cleared rapidly from the circulation by the liver.

TL;DR: A potential aldehyde group of an oligosaccharide is combined with 2-aminopyridine by means of the reductive amination with sodium cyanoborohydride giving a fluorescent 2-amines derivative, which can be used for the determination of a degree of polymerization, sequence of sugar units, and the preparation of finger prints.

TL;DR: It is concluded that concentrations of EGF equal to or less than those present in mouse plasma stimulate the resorption of mouse bone in organ culture by a mechanism that involves the enhanced local production of PGE2.

TL;DR: An activator protein of NAD kinase from the pea, Pisum satavum L., has been shown to be Ca2+-dependent and stimulates the activity of modulator protein dependent-cyclic nucleotide phosphodiesterase from porcine brain.

TL;DR: An improved technique has been developed which can quickly and easily unblock proteins and peptides containing pyrrolidone carboxylic acid (PCA) or pyroglutamic acid in the amino terminal position utilizing commercially available pyrog lutamate amino peptidase isolated from calf liver.

TL;DR: The substitutions Thr → Ala, Gln → Leu and Pro → Thr or Ala in mammalina α-crystallin A chains are found to increase the electrophoretic mobility in sodium dodecyl sulfate gel electrophoresis, able to detect neutral substitutions not usually visible in regular electrophoreis.

TL;DR: Pyridinoline is an amino acid isolated from collagen and probably serves as a crosslink in collagen fiber and is proposed on the basis of data investigated by 1 H-nmr and 13 C-nmR spectroscopy, mass spectroscopic and chemical degradation.

TL;DR: A thymidine kinase heterozygote designated H2BT has been isolated from the human lymphoblast line HH4 and significant increase in the trifluorothymidine-resistant fraction was observed in the new cell line following treatment with the mutagens ICR-191 and butylmethansulfonate.

TL;DR: It is concluded that this enzyme catalyzes the following reaction: Palmitoyl-CoA + O2 → transm -2-Hexadecenoyl, CoA + H2O2, which was most active toward C12-C18 acyl-CoAs.

TL;DR: The presence of normal levels of β-galactosidase activity in leukocytes from the mother of the patient indicates that the β-GalactOSidase deficiency is not the primary enzyme defect in this type of patient.

TL;DR: Aryl azides are rapidly reduced by dithiothreitol at room temperature to the corresponding aryl amines and the relevance of this reaction to experiments involving aryL azide photoaffinity reagents is discussed.

TL;DR: Several other 2-chloroethyl nitrosoureas were capable of inactivation of not only purified GSSG-reductase, but also the activity of this enzyme in cell-free extracts of murine lymphoma L5178Y ascites tumor cells and murine bone marrow.

TL;DR: It is indicated that prolyl hydroxylase can catalyze a number of reaction cycles without ascorbate, but at some stage thehydroxylation ceases, probably due to oxidation of the enzyme-bound iron, and ascorBate is then required as a quite specific reductant to re-activate the enzyme.

TL;DR: Atractyloside-insensitive binding of purine nucleotides is reduced in brown adipose tissue mitochondria of the obese ( ob ob ) mouse and it is suggested that the impaired thermogenesis of the ob ob mouse is due to a defect in this pathway in the mitochondria.

TL;DR: A pure straight chain ceramide hexasaccharide showed strong i-activity determined by hemagglutination inhibition and by radioimmunoassay with five out of six anti-i antisera and was abolished by elimination of the terminal Gal or by removal of the N-acetyl groups of the two GlcNAc residues.

TL;DR: The decline in β-adrenergic receptor numbers is not secondary to blood pressure elevation and may be related to increased sympathetic drive in spontaneously hypertensive rats.

TL;DR: Electron spin resonance measurements on aerated melanin suspensions during photoirradiation show changes in the microwave saturation of melanin free radicals and formation of adducts in the presence of spin traps, indicating that oxygen is reduced to superoxide and hydrogen peroxide.

TL;DR: The hydrogenase of the sulfate-reducer Desulfovibrio gigas has been purified to homogeneity and shows a specific activity of 90 μmoles H2 evolved/min./mg protein.

TL;DR: It is suggested that the role of the myosin light chain kinase is similar in both muscle and non-muscle cells and serves to activate the contractile apparatus, via the phosphorylation of myOSin, in response to an increase in the intracellular free Ca2+ concentration.

TL;DR: A high molecular weight protein aggregate, which agglutinates yeast cells, human epithelial cells and mouse lymphocytes, was isolated from extracts of Escherichia coli by differential centrifugation and gel filtration and showed that the lectin consists of protein subunits with identical Mr of ∼36500.

TL;DR: Multiple species of estrogen binding sites have been demonstrated in cytosol and nuclear fractions of uteri from immature and adult ovariectomized female rats and the presence of these sites in both cytosols and nuclear compartments complicates the accurate measurement and differentiation ofThese sites.

TL;DR: Observations indicate that intestinal chylomicron A apolipoproteins serve as precursors for plasma high density lipoprotein A apoledipoprotein and only a small fraction of chylomeron apoliprotein B is metabolized to form low density lipoipoprotein B.

TL;DR: Bromoacetyl[methyl-3H]choline is a highly specific label for the reduced acetylcholine binding site on the acetyl choline receptor from Torpedo californica as discussed by the authors.

TL;DR: The possible utility of vanadate sensitivity as a probe for dynein involvement in non-axonemal motile systems was explored with respect to brain ATPase associated with tubulin obtained by cycles of assembly, and ATPases associated with mitotic apparatus isolated from sea urchin embryos.