Tony P. Huang

TL;DR: Six optimized adenine base editors (ABEmax variants) are engineered that use SpCas9 variants compatible with non-NGG protospacer adjacent motifs that improves the targeting scope of cytosine and adenines base editing.

TL;DR: Three new SpCas9 variants that collectively recognize NRNH PAMs are reported that enable targeting of most NR PAM sequences and substantially reduce the fraction of genomic sites that are inaccessible by Cas9-based methods.

TL;DR: An extensive comparison of the PAM-sequence compatibilities and the on-target and off-target activities of Cas9 from Streptococcus pyogenes (SpCas9) and the Sp Cas9 variants xCas9 and SpCas9-NG and it is found that x Cas9 has the lowest tolerance for mismatched target sequences and that SpCas 9-NG has the broadest PAM compatibility.

TL;DR: An activity-independent form of SE-PACE is developed to correct folding-defective variants of maltose-binding protein (MBP) and to evolve variants of the eukaryotic cytidine deaminase APOBEC1 with improved expression properties, suggesting that SE- PACE can be applied to a wide variety of proteins to rapidly improve their soluble expression.

TL;DR: In this paper, the authors describe a protocol for using base editing in cultured mammalian cells and provide guidelines for choosing target sites, appropriate base editor variants and delivery strategies to best suit a desired application.