Tina Wang

TL;DR: Three new SpCas9 variants that collectively recognize NRNH PAMs are reported that enable targeting of most NR PAM sequences and substantially reduce the fraction of genomic sites that are inaccessible by Cas9-based methods.

TL;DR: It is shown that MBo is selective for MGO over other biologically relevant dicarbonyls and is suitable for detecting MGO in complex environments, including that of living cells, and its utility in estimating plasma concentrations of MGO is demonstrated.

TL;DR: It is reported that human MDSCs were characterized by strongly reduced metabolism and conferred this compromised metabolic state to CD8 + T cells, thereby paralyzing their effector functions and identifying the dicarbonyl methylglyoxal as a marker metabolite for MDSC that mediates T cell paralysis and can serve as a target to improve cancer immune therapy.

TL;DR: This article used ABE8e-NRCH to convert the β-globin allele (HBBS) into Makassar βglobin (HBBG), a non-pathogenic variant.

TL;DR: The existence of Lys and Arg modifications on histones derived from a glycolytic by-product, methylglyoxal (MGO), demonstrate the existence of a previously undetected histone modification and provide a link between cellular metabolism and the histone code.