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Peyton B. Randolph
Researcher at Howard Hughes Medical Institute
Publications - 8
Citations - 2829
Peyton B. Randolph is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 3, co-authored 3 publications receiving 1221 citations. Previous affiliations of Peyton B. Randolph include Harvard University & Broad Institute.
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Journal ArticleDOI
Search-and-replace genome editing without double-strand breaks or donor DNA
Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Jessie Rose Davis,Jessie Rose Davis,Jessie Rose Davis,Alexander A. Sousa,Alexander A. Sousa,Alexander A. Sousa,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Jonathan M. Levy,Jonathan M. Levy,Jonathan M. Levy,Peter J. Chen,Peter J. Chen,Peter J. Chen,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Aditya Raguram,Aditya Raguram,Aditya Raguram,David R. Liu,David R. Liu,David R. Liu +32 more
TL;DR: A new DNA-editing technique called prime editing offers improved versatility and efficiency with reduced byproducts compared with existing techniques, and shows potential for correcting disease-associated mutations.
Journal ArticleDOI
Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Shannon M. Miller,Tina Wang,Tina Wang,Tina Wang,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Mandana Arbab,Mandana Arbab,Mandana Arbab,Max W. Shen,Tony P. Huang,Tony P. Huang,Tony P. Huang,Zaneta Matuszek,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Holly A. Rees,Holly A. Rees,Holly A. Rees,David R. Liu,David R. Liu,David R. Liu +23 more
TL;DR: Three new SpCas9 variants that collectively recognize NRNH PAMs are reported that enable targeting of most NR PAM sequences and substantially reduce the fraction of genomic sites that are inaccessible by Cas9-based methods.
Journal ArticleDOI
Engineered pegRNAs improve prime editing efficiency
James W. Nelson,James W. Nelson,James W. Nelson,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Simon P. Shen,Simon P. Shen,Simon P. Shen,Kelcee A. Everette,Kelcee A. Everette,Kelcee A. Everette,Peter J. Chen,Peter J. Chen,Peter J. Chen,Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Meirui An,Meirui An,Meirui An,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Jonathan C. Chen,Jonathan C. Chen,Jonathan C. Chen,Alvin Hsu,Alvin Hsu,Alvin Hsu,David R. Liu,David R. Liu,David R. Liu +32 more
TL;DR: In this article, the 3′ terminus of pegRNAs was incorporated with 3′ RNA motifs to enhance their stability and prevent degradation of 3′ extension, which improved the efficiency of prime editing.
Journal ArticleDOI
Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins
Samagya Banskota,Aditya Raguram,Susie Suh,Samuel W. Du,Jessie Rose Davis,Elliot H. Choi,Xiao Wang,Sarah C. Nielsen,Gregory A. Newby,Peyton B. Randolph,Mark J. Osborn,Kiran Musunuru,Krzysztof Palczewski,David R. Liu +13 more
TL;DR: In this paper , the authors report the development and application of engineered DNA-free virus-like particles (eVLPs) that efficiently package and deliver base editor or Cas9 ribonucleoproteins.
Journal ArticleDOI
Designing and executing prime editing experiments in mammalian cells
TL;DR: In this article , the authors provide guidelines for selecting the proper PE system for a given application and how to perform PE in mammalian cells, as well as the design and optimization of pegRNAs.