T
Toshihiko Ashikari
Researcher at Kobe Gakuin University
Publications - 68
Citations - 3598
Toshihiko Ashikari is an academic researcher from Kobe Gakuin University. The author has contributed to research in topics: Yeast & Gene. The author has an hindex of 32, co-authored 68 publications receiving 3439 citations.
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Journal ArticleDOI
S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex
Yuki Katou,Yutaka Kanoh,Masashige Bando,Hideki Noguchi,Hirokazu Tanaka,Toshihiko Ashikari,Katsunori Sugimoto,Katsuhiko Shirahige +7 more
TL;DR: It is demonstrated that the checkpoint regulatory proteins Tof1 and Mrc1 interact directly with the DNA replication machinery in Saccharomyces cerevisiae, which forms a stable pausing structure that serves to anchor subsequent DNA repair events.
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Engineering of the Rose Flavonoid Biosynthetic Pathway Successfully Generated Blue-Hued Flowers Accumulating Delphinidin
Yukihisa Katsumoto,Masako Fukuchi-Mizutani,Yuko Fukui,Filippa Brugliera,Timothy A Holton,Mirko Karan,Noriko Nakamura,Keiko Yonekura-Sakakibara,Junichi Togami,Alix Pigeaire,Guo-Qing Tao,Narender S. Nehra,Chin-Yi Lu,Barry Dyson,Shinzo Tsuda,Toshihiko Ashikari,Takaaki Kusumi,John Mason,Yoshikazu Tanaka +18 more
TL;DR: This work down-regulated the endogenous dihydroflavonol 4-reductase (DFR) gene and overexpressed the Irisxhollandica DFR gene in addition to the viola F3'5'H gene in a rose cultivar, resulting in the accumulation of a high percentage of delphinidin and a novel bluish flower color.
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Genome Sequence of the Lager Brewing Yeast, an Interspecies Hybrid
Yoshihiro Nakao,Takeshi Kanamori,Takehiko Itoh,Yukiko Kodama,Sandra Rainieri,Norihisa Nakamura,Tomoko Shimonaga,Masahira Hattori,Toshihiko Ashikari +8 more
TL;DR: The genome sequence of this interspecies hybrid yeast provides a new tool for better understanding of lager brewing yeast behavior in industrial beer production.
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Pure and Mixed Genetic Lines of Saccharomyces bayanus and Saccharomyces pastorianus and Their Contribution to the Lager Brewing Strain Genome
Sandra Rainieri,Yukiko Kodama,Yoshinobu Kaneko,Kozaburo Mikata,Yoshihiro Nakao,Toshihiko Ashikari +5 more
TL;DR: This study identifies brewing strains that could be used as novel genetic sources in strain improvement programs and provides data that can be used to generate a model of how naturally occurring and industrial hybrid strains may have evolved.
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cDNA cloning, gene expression and subcellular localization of anthocyanin 5-aromatic acyltransferase from Gentiana triflora.
Hiroyuki Fujiwara,Yoshikazu Tanaka,Keiko Yonekura-Sakakibara,Masako Fukuchi-Mizutani,Masahiro Nakao,Yuko Fukui,Masa-atsu Yamaguchi,Toshihiko Ashikari,Takaaki Kusumi +8 more
TL;DR: Immunohistochemical analysis demonstrated that the 5AT protein is specifically expressed in the outer epidermal cells of gentian petals and that it is localized mainly in the cytosol, and this indicates that 5AT is a member of a proposed superfamily of multi-functional acyltransferases.