T
Toshiomi Yoshida
Researcher at Osaka University
Publications - 214
Citations - 6081
Toshiomi Yoshida is an academic researcher from Osaka University. The author has contributed to research in topics: Fermentation & Plasmid. The author has an hindex of 41, co-authored 214 publications receiving 5924 citations.
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Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells.
TL;DR: In order to get the high liquefaction yield from marine algae cell mass to fuel oil, the effect of salt stress on the accumulation of lipids and triacylglyceride in Dunaliella cells was investigated.
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Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999.
TL;DR: Limited feeding of nitrate during culture of Nannochloris sp.
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Stable expression of human β1,4-galactosyltransferase in plant cells modifies N-linked glycosylation patterns
Nirianne Marie Q. Palacpac,Shohei Yoshida,Shohei Yoshida,Hiromi Sakai,Yoshinobu Kimura,Kazuhito Fujiyama,Toshiomi Yoshida,Tatsuji Seki +7 more
TL;DR: The synthesis in plants of N-linked glycans with modified and defined sugar chain structures similar to mammalian glycoproteins is demonstrated, demonstrating the success of the plant cell N-glycosylation pathway.
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Construction of xylose-assimilating Saccharomyces cerevisiae
TL;DR: The transformants of S. cerevisiae harboring the resultant plasmids produced xylose reductase constitutively at a rate about 3 times higher than P. stipitis, but could not assimilatexylose due to the deficient conversion of xylitol to xylulose.
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Two Distinct Mechanisms Cause Heterogeneity of 16S rRNA
TL;DR: To investigate the frequency of heterogeneity among the multiple 16S rRNA genes within a single microorganism, direct sequencing patterns revealed the existence of 136 heterogeneous loci among a total of 33 strains, suggesting that at least two mechanisms, possibly misincorporation during DNA replication and horizontal gene transfer, cause rRNA heterogeneity.