Valeria de Turris

TL;DR: This study uses dual-color single-molecule RNA imaging in living human cells to construct a complete kinetic profile of transcription and splicing of the β-globin gene and finds that kinetic competition results in multiple competing pathways for pre-mRNA splicing.

TL;DR: It is shown that aberrant localization and recruitment of FUS into stress granules (SGs) is a prerogative of the FUS mutant proteins and occurs only upon induction of stress in both undifferentiated iPSCs and spinal cord neural cells.

TL;DR: It is reported that relevant targets of miR-375, including the neural RNA-binding protein ELAVL4 and apoptotic factors, are aberrantly increased in FUS mutant motoneurons.

TL;DR: A non-contact and label-free imaging method, named background-deflection Brillouin microscopy, is presented, enabling the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells at a sub-micron resolution.

TL;DR: The RNA interactome of wild-type and mutant FUS in human motor neurons (MNs) is reported and it is proposed that pathological mutations in FUS trigger an aberrant crosstalk with ELAVL4 with implications for ALS.