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Daniel R. Larson
Researcher at National Institutes of Health
Publications - 74
Citations - 11572
Daniel R. Larson is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Gene & Transcription (biology). The author has an hindex of 33, co-authored 68 publications receiving 10379 citations. Previous affiliations of Daniel R. Larson include Howard Hughes Medical Institute & Cornell University.
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Precise nanometer localization analysis for individual fluorescent probes
TL;DR: A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation.
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Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.
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Bright and Stable Core−Shell Fluorescent Silica Nanoparticles
TL;DR: These nanoparticles are monodisperse in solution, 20 times brighter, and more photostable than their constituent fluorophore, and are amenable to specific labeling of biological macromolecules for bioimaging experiments.
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Single-RNA counting reveals alternative modes of gene expression in yeast.
TL;DR: Combining single-transcript measurements with computational modeling indicates that low expression variation is achieved by transcribing genes using single transcription-initiation events that are clearly separated in time, rather than by transcriptional bursts.
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Real-time observation of transcription initiation and elongation on an endogenous yeast gene.
Daniel R. Larson,Daniel Zenklusen,Daniel Zenklusen,Bin Wu,Jeffrey A. Chao,Robert H. Singer,Robert H. Singer +6 more
TL;DR: A method of fluctuation analysis of fluorescently labeled RNA is described to measure dynamics of nascent RNA—including initiation, elongation, and termination—at an active yeast locus and finds no transcriptional memory between initiation events, and elongation speed can vary by threefold throughout the cell cycle.