Valerie Zacny

TL;DR: The human papillomavirus E7 protein plays an important role in the viral life cycle by subverting the tight link between cellular differentiation and proliferation in normal epithelium, thus allowing the virus to replicate in differentiating epithelial cells that would have normally withdrawn from the cell division cycle.

TL;DR: Results indicate the close functional association of the E2 and E1 proteins in the alphavirus spike and have implications for the design of recombinant live virus vaccines for VEE, for otherAlphaviruses, and for other viruses that use a similar mechanism for glycoprotein maturation.

TL;DR: HPV-16 E7-expressing keratinocytes were more prone to both TNF-α- and TRAIL-mediated apoptosis compared with vector-infected controls, and both cytokines induced apoptosis when administered in combination with the protein synthesis inhibitor cycloheximide, but the apoptotic response to TRAIL was significantly more rapid and efficient compared with the response seen after TNP treatment.

TL;DR: It is shown that in primary cultures of human foreskin keratinocytes, TNFalpha mediates cellular growth arrest through activation of the transcription factor NF-kappaB, and the cdk inhibitor p21(Cip1/Waf1) is activated through NF- kappaB and is an important mediator of this growth arrest response.

TL;DR: It is shown that normal human diploid fibroblasts that express the human papillomavirus type 16 E7 oncoprotein have a decreased propensity to undergo apoptosis in response to TNF treatment and the ability of E7 to undermine TNF-mediated apoptosis correlates with cellular transformation.