Jonathan F. Smith

TL;DR: The VEE replicon system was characterized by high-level expression of heterologous genes in cultured cells, little or no regeneration of plaque-forming virus particles, the capability for sequential immunization to multiple pathogens in the same host, and induction of protective immunity against a mucosal pathogen.

TL;DR: Using standard histologic and ultrastructural methods, virus isolation, immunohistochemistry, in situ hybridization and reverse-transcriptase polymerase chain reaction,West Nile virus was identified as the cause of clinical disease, severe pathologic changes, and death in 27 birds representing eight orders and 14 species and should aid diagnosticians faced with the emergence of West Nile virus in the United States.

TL;DR: A molecular clone of Venezuelan equine encephalitis virus (VEE) was constructed from four cDNAs that were synthesized using the viral RNA genome as template.

TL;DR: An RNA replicon, based upon Venezuelan equine encephalitis (VEE) virus, was used as a vaccine vector; the VEE structural genes were replaced by genes for MBGV GP, nucleoprotein (NP), VP40, VP35, VP30, or VP24.

TL;DR: The 74HB59 strain of Rift Valley fever virus, isolated from a human case in the Central African Republic, was shown to be composed of a heterogeneous population of viruses when plaque-purified clones were analyzed for their reactivity with monoclonal antibodies directed against the nucleocapsid (N) protein or the nonstructural (NSs) protein.