Vanya D. Uzunova

TL;DR: A new design criterion for aqueous synthetic receptors has therefore emerged, which is the optimization of the size of cavities and binding pockets with respect to the energy and number of residing water molecules.

TL;DR: The combined results indicate a sufficiently low toxicity of cucurbit[n] as additives for medicinal and pharmaceutical use, and the bioadaptability of the compounds was further examined through in vivo studies on mice.

TL;DR: The described applications bypass problems related to the unselective binding of the macrocycle by coupling the signalling event with highly specific enzymatic transformations and the highly selective determination of absolute, low micromolar concentrations of both choline and acetylcholine by simple fluorescence measurements.

TL;DR: The extension of the high selectivity and muM sensitivity of the tandem assay principle has allowed for the accurate measurement of D-lysine enantiomeric excesses of up to 99.98 %, as only the L-enantiomer is accepted by the enzyme as a substrate and is converted to the product that is responsible for the observed fluorescence signal.

TL;DR: The binding affinity of Neutral Red with cucurbit[7]uril (CB7) can be fine-tuned by addition and competitive binding of metal ions, which leads to a pK(a) shift of the dye; this can be exploited to relocate the dye from the macrocyclic cavity of CB7 to the biomolecular pocket of bovine serum albumin.