Venkata Ragireddy

TL;DR: The results indicate that human mARC-1 and m ARC-2 are capable of catalyzing reduction of nitrite to NO through reaction with its molybdenum cofactor, and indicate that mARC can generate nitric oxide from nitrite when forming an electron transfer chain with NADH, cytochrome b5, and NADH-dependent cyto chrome b5 reductase.

TL;DR: Using knockdown of all molybdopterin enzymes and SO in fibroblasts isolated from patients with genetic deficiencies of molybdenum cofactor and SO, SO was found to significantly contribute to hypoxic nitrite signaling as demonstrated by activation of the canonical NO-sGC-cGMP pathway.

TL;DR: A relationship between the nitrite reduction rate and heme accessibility in Ngb, particularly marked for His64(E7) mutants is suggested, which may be related to a differential stabilization of the iron–nitrite complexes in five- and six-coordinate globins.

TL;DR: It is established that mARC can generate NO from nitrite in the presence of NADH, cytochrome b5, and cyto chrome b5 reductase at pH 7.4 and suggested that cysteine 270 and molybdenum are important in the transformation of nitrite to NO.