Victor Venegas

TL;DR: It is found that satellite glial cell precursors are the primary phagocytic cells for apoptotic corpse removal in developing mouse dorsal root ganglia (DRG), and Jedi-1 and MEGF10 are identified as homologs of the invertebrate engulfment receptors Draper and CED-1 expressed in the glial precursor cells.

TL;DR: This chapter outlines the real-time quantitative polymerase chain reaction (qPCR) analysis of mtDNA content in muscle or liver tissues and outlines the nine genes reported to cause various forms of MDDSs.

TL;DR: Observations suggest that phosphatidylserine might be recognized by CED-1 and act as a conserved eat-me signal from nematodes to mammals, and the two different biochemical activities used in somatic cells (ABC transporter) and germ cells (phospholipid scramblase) suggest an increased complexity in the regulation of phosphatidoserine presentation in response to apoptotic signals in different tissues and during different developmental stages.

TL;DR: This unit outlines the real‐time quantitative polymerase chain reaction (qPCR) analysis of mtDNA content in tissues and describes protocols for the selection of nuclear genes for normalization, PCR set up, validation procedures, tissue and age matched controls, and sensitivity and specificity in various tissues, as well as interpretation of results.

TL;DR: The combined use of biochemical and biophysical approaches indicated that this functional cooperation relies on the alternate association of these receptors with a common partner, endogenously expressed in the cell system, which is the first working model structuring in mammals the CED-1 dependent pathway.