Vikram Chaudhery

TL;DR: Dose-response characterization of the photonic crystal antibody microarrays shows the capability to detect common cancer biomarkers in the <2 pg/mL concentration range within a mixed sample.

TL;DR: PCEM provides rich, dynamic information about the evolution of cell-surface attachment profiles over biologically relevant time-scales and retains the ability to monitor cell behavior with spatial resolution sufficient for observing both attachment footprints of filopodial extensions and intracellular attachment strength gradients.

TL;DR: An optical imaging system capable of exciting surface-bound fluorophores within the resonant evanescent electric field of a photonic crystal surface and gathering fluorescence emission that is directed toward the imaging objective by the photonic crystals is reported on.

TL;DR: A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500x for the detection of LD-700 dye spin-coated upon the PC.

TL;DR: This work demonstrates the first silicon-based PCEF detection platform for multiplexed biomarker assay, and designs a compact detection instrument designed to efficiently couple fluorescence excitation from a semiconductor laser to the resonant optical modes of the PC, resulting in elevated electric field strength.