Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection.
Anusha Pokhriyal,Meng Lu,Vikram Chaudhery,Cheng-Sheng Huang,Stephen C. Schulz,Brian T. Cunningham +5 more
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TLDR
A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500x for the detection of LD-700 dye spin-coated upon the PC.Abstract:
A Photonic Crystal (PC) surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated to enhance light emission from fluorescent molecules in close proximity to the PC surface. Quartz was selected for its low autofluorescence characteristics compared to polymer-based PCs, improving the detection sensitivity and signal-to-noise ratio (SNR) of PC Enhanced Fluorescence (PCEF). Nanoimprint lithography enables economical fabrication of the subwavelength PCEF surface structure over entire 1x3 in2 quartz slides. The demonstrated PCEF surface supports a transverse magnetic (TM) resonant mode at a wavelength of λ = 632.8 nm and an incident angle of θ = 11°, which amplifies the electric field magnitude experienced by surface-bound fluorophores. Meanwhile, another TM mode at a wavelength of λ = 690 nm and incident angle of θ = 0° efficiently directs the fluorescent emission toward the detection optics. An enhancement factor as high as 7500 × was achieved for the detection of LD-700 dye spin-coated upon the PC, compared to detecting the same material on an unpatterned glass surface. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330 × SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140 × lower limit of detection compared to a conventional glass substrate.read more
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Photonic crystals: Emerging biosensors and their promise for point-of-care applications
Hakan Inan,Muhammet Poyraz,Fatih Inci,Mark A. Lifson,Murat Baday,Brian T. Cunningham,Utkan Demirci +6 more
TL;DR: A broad overview of PCs is provided by explaining their structures, fabrication techniques, and sensing principles, and recent applications of PC-based biosensors incorporated with emerging technologies, including telemedicine, flexible and wearable sensing, smart materials and metamaterials.
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Recent Advances in Resonant Waveguide Gratings
TL;DR: In this paper, the authors discuss the latest developments in the field of Resonant Waveguide gratings (RWGs), including numerical modeling, manufacturing, the physics, and applications of RWGs, and links to the standard tools and references in modeling and fabrication according to their needs.
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Enabling enhanced emission and low-threshold lasing of organic molecules using special Fano resonances of macroscopic photonic crystals
Bo Zhen,Song Liang Chua,Jeongwon Lee,Alejandro W. Rodriguez,Xiangdong Liang,Steven G. Johnson,John D. Joannopoulos,Marin Soljacic,Ofer Shapira +8 more
TL;DR: A unique optofluidic platform comprising organic molecules in solution suspended on a photonic crystal surface, which supports macroscopic Fano resonances and allows strong and tunable interactions with the molecules anywhere along the surface is presented.
Journal ArticleDOI
Two-dimensional photonic crystals for sensitive microscale chemical and biochemical sensing
TL;DR: This review focuses on one class of photonic crystal, in which light is confined to a patterned planar material layer of sub-wavelength thickness, suitable for incorporation into lab-on-a-chip systems and in theory can provide exceptional sensitivity.
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Optimally designed narrowband guided-mode resonance reflectance filters for mid-infrared spectroscopy
TL;DR: Optimal design of double-layer surface-relief silicon nitride-based GMR filters in the mid-IR for various narrow bandwidths below 32 cm−1 is presented, which will enable use ofGMR filters-based instrumentation for common measurements of condensed matter, including tissues and polymer samples.
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