Virginia Maria Grazia Borrelli

TL;DR: Recently, CRISPR/Cas9 has largely overtaken the other genome editing technologies due to the fact that it is easier to design and implement, has a higher success rate, and is more versatile and less expensive.

TL;DR: Findings from transcriptomic studies have been used to outline a specific model for the intracellular signaling cascade occurring in maize cells against F. verticillioides infection, and several quantitative trait loci and single-nucleotide polymorphism markers for resistance to Fusarium ear rot deriving from QTL mapping and genome-wide association studies are described, indicating the complexity of this polygenic trait.

TL;DR: The analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR–Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of single and multiple knockouts.

TL;DR: Genome Wide Association Study of traits related to Fusarium seedling rot was carried out and linkage disequilibrium analysis revealed an additional gene not directly observed by GWAS analysis, which could aid to better understand the complex interaction between maize and F. verticillioides.

TL;DR: In this paper, the functional characterization of the 9-LOX gene, ZmLOX4, was carried out by employing mutants carrying Mu insertions in this gene (named as UFMulox4).