V
Vladimír Sklenář
Researcher at Central European Institute of Technology
Publications - 107
Citations - 7180
Vladimír Sklenář is an academic researcher from Central European Institute of Technology. The author has contributed to research in topics: Nuclear magnetic resonance spectroscopy & Chemical shift. The author has an hindex of 35, co-authored 107 publications receiving 6952 citations. Previous affiliations of Vladimír Sklenář include Masaryk University & Czechoslovak Academy of Sciences.
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Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.
TL;DR: A novel approach to tailored selective excitation for the measurement of NMR spectra in non-deuterated aqueous solutions, which effectively combines one selective 180° radiofrequency pulse and two field gradient pulses, achieves highly selective and effective water suppression.
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Spin-echo water suppression for the generation of pure-phase two-dimensional NMR spectra
Vladimír Sklenář,Ad Bax +1 more
TL;DR: In this article, a new approach for water suppression in one and two-dimensional NMR, generating absorption-mode spectra that are free of baseline distortions, is described, which involves the use of a 1−1 hard pulse as a read pulse, followed by a 1 −1 refocusing pulse which is phase cycled to obtain the greatest possible water suppression.
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Localization of ammonium ions in the minor groove of DNA duplexes in solution and the origin of DNA A-tract bending.
TL;DR: The sequence-specific nature of monovalent cation binding sites demonstrated here for A-tract DNA provides an explanation for the origin of sequence-directed bending.
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Binding sites and dynamics of ammonium ions in a telomere repeat DNA quadruplex.
TL;DR: The 15NH4+ is used as a probe of cation coordination by the four G quartets of the DNA bimolecular quadruplex [d(G4T4G4], formed from oligonucleotides with the repeat sequence found in Oxytricha nova telomeres, and is shown to be a valuable probe of monovalent cation binding sites and dynamics.
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DNA A-tract bending in three dimensions: Solving the dA4T4 vs. dT4A4 conundrum
TL;DR: Results of gel electrophoretic mobility studies are consistent with the higher-order structure of the DNA and furthermore depend on the nature of the monovalent cation present in the running buffer.