Showing papers by "Vojtěch Adam published in 2009"

Detection of metallothionein and alpha-methylacyl CoA racemaseas potential new markers for prostate carcinoma

Abstract: In many developed countries, prostate cancer is the second leading cause of cancer-related deaths among men. Therefore, having reliable and easy-to-detect marker is needed to treat this disease successfully. Metallothioneins (MT) are a group of proteins rich in cysteine with molecular weights ranging from 6 to 10 kDa. Their main physiological functions are homeostatic control and detoxification of the metals. The role of MT in tumour tissue remains still unclear, but MT can be considered as new promising tumour marker. Besides MT, alpha-methylacyl CoA racemase AMACR has been proven to be one of the few biomarkers that can help distinguish cancer from benign cells, with high sensitivity and specificity for prostate carcinoma. In this study we proposed new methodical approaches in MT and AMACR detection in cell line model and then reciprocal correlation of obtained data. The LNCaP cell line used in our experiments was isolated in 1977 by J.S. Horoszewicz, et al. An adsorptive transfer stripping technique coupled with differential pulsed voltammetry Brdicka reaction was employed for the determination of metallothionein MT and AMACR level in cell lines extract. MT content was hundred of ug per g of soluble proteins and was higher as well as for AMACR compared to non-tumour cell line.

Doxorubicin and genomic DNA, new insights in drug-nucleic acidsinteractions

Abstract: Anthracyclines including doxorubicin are commonly used in the treatment of a number of diverse malignant. There is compelling evidence that cellular DNA is the primary target for anthracyclines. In this study we aimed at investigation of intercalation of doxorubicin into DNA in vitro by using of square wave voltammetry coupled with adsorptive transfer stripping technique. Based on the decline of the signal of adenine and cytosine, we indicated binding of doxorubicin into dsDNA. The electrochemical method was further utilized for detection of doxorubicin-DNA adducts in neuroblastoma cells. Prior to electrochemical analysis dsDNA was carefully isolated from these cells to avoid modification of DNA bases. We observed significant (a=0.05) difference in DNA signals after intercalation of doxorubicin between resistant and sensitive cells. For more comprehensive understanding of the intercalation, computational experiments using design for testability approach with correction on dispersion interaction were performed. Several molecular structures were explored and clear preference for very stable stacked structures was observed.

Electrochemical and computational study of doxorubicininteractions with DNA

Abstract: This work deals with doxorubicin interactions with DNA which were studied by means of electrochemistry and computational modelling

Comparison of electrochemical and spectrometric detection of-SHmoieties

Abstract: This study was focused on spectral and electrochemical detection of thiols and glutathione. Results obtained by both methods showed very good correlation.

Paramagnetic particles with specific sequences of nucleic acidsas tools for detection heavy-metal-stress-induced genes'expression in plants

Abstract: We isolated DNA from spruce and maize embryos which were treated by Cd(II) ions by means of paramagnetic particles.

Mapping of nutrition elements and heavy metals in plant tissue slices by laser-induced breakdown spectroscopy

Abstract: Double pulse LIBS was utilized for mapping the nutrition elements and the accumulation of the heavy metals in a plant tissue slices. Elemental maps obtained by this technique were compared with images from fluorescence microscopy.